酸敏感离子通道在大鼠岩神经节神经元的表达

Expression of Acid-sensing Ion Channels in Petrosal Ganglion

目的:探讨酸敏感离子通道(acid-sensing ion channels,ASICs)的四种亚型即ASIC1a、ASIC1b、ASIC2a和ASIC3是否表达于大鼠岩神经节(petrosal ganglion)神经元。方法:采用常规免疫组化法(PV法),观察正常大鼠岩神经节神经元是否表达ASIC1a、ASIC1b、ASIC2a和ASIC3。结果:在正常大鼠岩神经节神经元,可见ASIC1a、ASIC1b、ASIC2a和ASIC3表达;ASIC1a与ASIC2a,ASIC3与ASIC1b,ASIC3与ASIC1a,ASIC3与ASIC2a在岩神经节神经元上共表达。结论:正常生理情况下,大鼠岩神经节神经元均表达ASIC1a、ASIC1b、ASIC2a和ASIC3;ASICs亚型之间的共表达提示,在岩神经节可能存在ASICs异聚体的方式。

Objective： To investigate the expression of four subtypes of acid-sensing ion channels（ASICs）,ASIC1a,ASIC1b,ASIC2a and ASIC3,of the neurons in the petrosal ganglions of rats.Methods： The expression of ASIC1a,ASIC1b,ASIC2a and ASIC3 of the neurons in the petrosal ganglions of rats was investigated with the immunohistochemical technique.Results： The ASIC1a-,ASIC1b-,ASIC2a-and ASIC3-positive immunoreactive neurons were all observed in the petrosal ganglions of rats.The co-expressions of ASIC1a and ASIC2a,ASIC3 and ASIC1a,ASIC3 and ASIC1b,ASIC3 and ASIC2a on a same neuron were observed separately.Conclusion： The ASIC1a,ASIC1b,ASIC2a and ASIC3 exist in the neurons of the petrosal ganglions in normal rats.Co-expression of different subtypes existing in the same neuron suggests that ASICs may exist in the form of heteromer in the petrosal ganglion.