蜂胶水提液对人脐动脉平滑肌细胞增殖作用的影响

Effect of Water Extract Propolis on Human Umbilical Artery Smooth Muscle Cell Proliferation

目的观察蜂胶水提液对血管紧张素Ⅱ诱导的人脐动脉平滑肌细胞增殖作用的影响。方法贴块法培养人脐动脉平滑肌细胞,用50、100和200 mg/L蜂胶水提液进行干预。细胞计数法检测人脐动脉平滑肌细胞数量;流式细胞仪检测细胞周期;免疫细胞化学法观察增殖细胞核抗原;分光光度计检测培养液中超氧化物歧化酶及丙二醛的含量;用脱氧核糖核苷酸末端转移酶介导的缺口末端标记技术检测细胞凋亡。结果100 mg/L和200mg/L的蜂胶水提液能减少血管紧张素Ⅱ对细胞的增殖作用,增加G_0/G_1期细胞的百分比,降低增殖细胞核抗原阳性率,蜂胶各浓度组总超氧化物歧化酶活力升高,而丙二醛含量降低,凋亡指数降低(P<0.01)。结论一定浓度的蜂胶水提液能抑制血管紧张素Ⅱ诱导的人脐动脉平滑肌细胞增殖;机制可能与蜂胶水提液影响细胞增殖周期、抑制增殖细胞核抗原的表达、提高人脐动脉平滑肌细胞抗氧化能力有关。

Aim To evaluate the effect of water extract propolis （WEP） on human umbilical artery smooth muscle cell proliferation （HUASMC） induced by angiotensin Ⅱ （ Ang Ⅱ ） in vitro and explore the underlying mechanism. Methods HUASMC were cultured by the explant method and incubated with 50 mg/L, 100 mg/L and 200 mg/L WEP. HUASMC number was assessed by cell counting; Flow cytometry was used to test cell cycle; Proliferating cell nuclear antigen（ PCNA） of HUASMC was detected by immunocytochemistry staining; Superoxide dismutase （SOD） and maleic dialdehyde （MDA） in HUASMC culture medium superior was measured with spectrophotometer; Apoptosis was evaluated by terminal-deoxynucleotidyl transferase mediated nick end labeling （TUNEL）. Results Compared to model group, the numbers , PCNA expression,MDA, Apoptosis Index of 100 mg/L and 200 mg/L WEP were lower（ P 〈 0.01 ）, while the Percentage of the Cells in G0/G1 phase and SOD was higher（P 〈 0.01）. Conclusion Certain concentration of WEP can prevent HUASMC proliferation effect mediated by Ang Ⅱ ; the mechanism about WEP effect may be associated with arresting G1 to S progression, decreasing PCNA expression and improving the antioxidation of HUSMC.