临床分离103株溶血葡萄球菌中耐甲氧西林菌株的耐药性检测

Meticillin-resistant Staphylococcus haemolyticus and Resistance in 103 Isolates of S.haemolyticus

目的了解临床分离溶血葡萄球菌中耐甲氧西林溶血葡萄球菌(MRSH)的发生率及其对常用抗菌药物的耐药性。方法采用头孢西丁纸片法检测MRSH,琼脂稀释法测定MRSH和MSSH(甲氧西林敏感溶血葡萄球菌)对多种抗菌药物的敏感性。结果溶血葡萄球菌中MRSH的发生率为86.4%,其对青霉素、头孢唑林、头孢呋辛、头孢曲松、四环素、环丙沙星、克林霉素的耐药显著,对万古霉素、替考拉宁耐药率为0,对阿米卡星、利福平、氯霉素的耐药率分别为16.9%、11.2%、28.1%,除阿米卡星、四环素、利福平和氯霉素外,MRSH对其他抗菌药物的耐药率显著高于MSSH,差异有统计学意义。结论临床分离溶血葡萄球菌中,MRSH发生率较高、多药耐药显著,应加强检测和监测。

OBJECTIVE To investigate the prevalence of meticillin-resistant Staphylococcus haemolyticus （MRSH） and resistance in S. haemolyticus isolates. METHODS MRSH was detected by cefoxitin disc test and susceptibilities were tested by agar dilution method. RESULTS There were 86.4% of MRSH isolates. MRSH was highly resistant to penicillin, cefazolin, cefuroxime, ceftriaxone, tetracycline, eiprofloxacin, and clindamycin. The resistance rate to amikacin, rifampicine and chloramphenicol was 16.9%, 11.2% and 28. 1%, respectively. All isolates were susceptible to vancomycin and teicoplanin. Except for tetracycline, amikacin, rifampicin, and chloramphenicol, the resistant rate to other antimicrobial agents was significantly higher in MRSH than in MSSH. CONCLUSIONS MRSH is mostly occupied in S. haemolyticus isolates and resistant to most of antimicrobial agents. More attention should be paid to survey and detect these strains.