早期药膳饮食对烫伤大鼠肠黏膜Peyer’s结T淋巴细胞及亚群的影响

Earlier period medicated diet influences intestinal mucosa T lymphocyte of Peyer’s patch and T-lymphocyte subsets in severe scalded rats

背景:肠相关淋巴组织的组成部分Peyer’s结是肠黏膜免疫系统的重要诱导部位,含有参与免疫应答的各种免疫细胞,如CD4+T细胞、CD8+T细胞、B细胞等。目的:观察早期药膳饮食对烫伤大鼠肠道肠黏膜Peyer’s结T淋巴细胞及其亚群的影响。设计、时间及地点:随机对照动物实验,于2006-04/2007-03在南昌大学一附属医院完成。材料:Wistar大鼠100只,随机分成药膳喂养组(n=30)、肉汤喂养组(n=30)、常规喂养组(n=30)、正常对照组(n=10)。药膳成分:党参20g、白术25g、茯苓25g、炙甘草15g、黄芪20g、薏苡仁20g、瘦猪肉200g。方法:药膳喂养组、肉汤喂养组、常规喂养组实施背部30%体表面积Ⅲ度烫伤,伤后早期分别给予药膳、肉汤和生理盐水,2mL/次,2次/d。正常对照组实施37℃假伤。主要观察指标:伤后3,7,14d检测大鼠肠黏膜Peyer’s结T淋巴细胞及亚群。结果:伤后3,7d,药膳喂养组、肉汤喂养组、常规喂养组Peyer’s结淋巴细胞总数、CD3+淋巴细胞的百分比及总数、CD4+百分比及总数、CD8+细胞的百分比及总数较正常对照组明显减少(P<0.05,P<0.01),至14d,药膳喂养组与正常对照组相比差异不显著。伤后3,7,14d药膳喂养组CD3+淋巴细胞的百分比及总数、CD4+百分比及总数较肉汤喂养组、常规喂养组明显升高(P<0.05)。伤后3,7d药膳喂养组Peyer’s结CD8+细胞的百分比及总数较肉汤喂养组、常规喂养组明显下降(P<0.05)。结论:早期药膳饮食有利于肠道Peyer’s结淋巴细胞的增生,并改善Peyer’s结T淋巴细胞亚群的比例,从而改善烫伤大鼠肠道免疫功能。

BACKGROUND： Peyer’s patch of gut-associated lymphoid tissue is a important locus of intestinal mucosa immune system, which contains various kinds immunocyte to participate immune response, such as CD4＋ T lymphocyte, CD8＋ T lymphocyte, B lymphocyte and so on. OBJECTIVE： To observe the effects of T lymphocyte cells and subsets of rat Peyer’s patch on the intestinal mucosa by earlier oral feed with medicined diet in severe scalded rats. DESIGN, TIME AND SETTING： A randomized controlled animal experiment was performed at the First Affiliated Hospital of Nanchang University between April 2006 and March 2007. MATERIALS： One hundred Wistar rats were randomly divided into the medicined diet group （n=30）, bouillon group （n=30）, convention group （n=30） and the normal control group （n=10）. The medicined diet contains： codonopsis 20 g; atractylodes macrocephala 25 g; hoelen 25 g; prepared liauorice root 15 g; milkvetch root 20 g; coix seed 20 g; and lean pork 200 g. METHODS： Rats were subjected to 30% TBSAⅢ°scald on the back and followed by feed with medicined diet, bouillon and physiological saline with 2 mL per order for 2 times per day, respectively. Rats of the normal control group were received fake burned in 37 ℃ MAIN OUTCOME MEASURES： Changes of T lymphocyte cells and subgroups of rat intestinal Peyer’s patch were detected at days 3, 7, and 14 after operation. RESULTS： The total lymphocyte count of Peyer’s patch, percentage and total count of CD3＋, CD4＋ and CD8＋ cells in the medicined diet, bouillon and convention groups were obvious decreased than normal control group at days 3 and 7 （P 〈 0.05, P 〈 0.01）. However, there was no significant difference between the medicined diet group and normal control group at days 14. The percentage and total count of CD3＋, CD4＋ in the medicined diet was notably increased than that of the bouillon and convention groups at days 3, 7, and 14 （P 〈 0.05）. Percentage and total count of CD8＋ cells in the medicined diet was noticeably decreased than that of the bouillon and convention groups at days 3, and 7 （P 〈 0.05）. CONCLUSION： The earlier period medicined diet can accelerate the hyperplasy of Peyer’s patch cell in the intestinal tract,improve the proportion of T-lymphocyte subsets, and eventually improve the immunity function of intestinal tract.