广西壮族人群转化生长因子β1基因多态性与腰椎间盘突出症血瘀证的关联性

Association of transforming growth factor beta 1 gene polymorphism with blood stasis syndrome following lumbar intervertebral disc protrusion in Zhuang population from Guangxi

目的:观察腰椎间盘突出症血瘀证与转化生长因子β1基因多态性的相关性。方法:筛选符合标准的广西壮族人群腰椎间盘突出症患者88例,分为血瘀证组30例与非血瘀证组58例,进行转化生长因子β1基因单核苷酸多态性(SNP)检测,观察中医证型与SNP的关联性。结果:转化生长因子β1基因-800位点基因型在血瘀证组与非血瘀证组间分布差异无显著性意义(P>0.05),转化生长因子β1基因-509位点基因型在血瘀证组与非血瘀证组间分布差异有显著性意义(P<0.05),Logistic回归分析结果显示,与转化生长因子β1基因-509位点TT基因型相比,携带转化生长因子β1基因-509位点CT基因型者患血瘀证的风险较高(P<0.05)。结论:转化生长因子β1基因-509C/T多态性与腰椎间盘突出症血瘀证相关。

OBJECTIVE： To investigate the relationship between the blood stasis syndrome of lumbar intervertebral disk protrusion and transforming growth factor β1 （TGF-β1） gene polymorphisms. METHODS： A total of 88 up to standard patients with lumbar intervertebral disk protrusion from Guangxi Zhuang Autonomous Region were screened, with 30 ones with blood stasis syndrome and the other 58 ones without. Then, the single nucleotide polymorphism （SNP） of TGF-β1 was tested to investigate the relationship between the traditional Chinese medicine syndrome and the SNP. RESULTS： Difference of significance existed in the genotype distribution of TGF-β1 -509 site （P 〈 0.05） rather than TGF-β1 -800 site （P 〉0.05） between blood stasis syndrome group and non-blood stasis syndrome group. It was showed by logistic regression analysis that the prevalence of blood stasis syndrome was higher for carriers of CT genotype in TGF-β1 -509 site than those of TT genotype （P 〈 0.05）. CONCLUSION： TGF-β1 gene -509C/T gene polymorphisms is relevant to the blood stasis syndrome of lumbar intervertebral disk protrusion.