对羟基苯乙基-α-D-葡萄糖甙对小鼠天然杀伤细胞的体外影响

Effect of phydroxyphenethylαDglucoside on natural killer cell in mice in vitro

本文用［３Ｈ］ＴｄＲ靶细胞释放法，观察了对羟基苯乙基－α－Ｄ－葡萄糖甙在体外对小鼠脾天然杀伤细胞（ＮＫ）活性的影响．结果表明，对羟基苯乙基－α－Ｄ－葡萄糖甙能提高正常鼠的脾ＮＫ杀伤活性，显著提高环磷酰胺给药后的ＮＫ杀伤活性，减轻由地塞米松引起的ＮＫ活性低下，协同白细胞介素－２明显增强鼠脾细胞的ＮＫ活性，而且剂量与作用呈正比．

The effect of phydroxyphenethylαDglucoside pHPG(α)] isolated from the fruits of Ligustrum lucidum Ait. on natural killer(NK) cell activity in vitro was studied by TdR target release assay. When murine splenocytes were cultured with pHPG(α) at various concentrations (50, 25 and 5 μg·L1) alone or combined with interleukin2. The cytotoxicity of NK was significantly increased. pHPG(α) also had the ability to protect the NK activity from the inhibition induced by cyclophosphamide and dexamethasone, especially by cyclophosphamide. These results suggest that pHPG(α) is one of the water soluble compounds posssessing immunomodulating effect in Ligustrum lucidum Ait.