黑曲霉B0201利用五倍子固体发酵产单宁酶的条件研究

Research on Conditions of Tannase Production from Aspergillus Niger B0201 Under Solid-state Fermentation Using Gallnut

通过比较常规灭菌发酵和生料发酵,研究黑曲霉B0201利用五倍子固体发酵产单宁酶的条件。结果表明,在生料发酵过程中,采用20%五倍子并且以(NH4)2SO4为氮源制备单宁酶的最佳条件为:液固比=1.6:1、温度30°C、初始pH6.0。在该条件下通过96h的培养,单宁酶的活力可达51.2U/gds,是常规灭菌发酵的3.6倍。以上结果显示,生料发酵生产单宁酶是一种高效可行的方法。

According to the comparative research of conventional sterile fermentation and uncooked material fermentation, the conditions of tannase production from Aspergillus Niger B0201 under solid-state fermenta- tion using gallnut was studied. The results showed that the liquid-solid ratio of 1.6： 1, temperature of 30℃, initial pH of 6.0 were the optimal conditions for tannase production under uncooked material fermentation with gallnut content of 20% and （NH4）2SO4 for nitrogen sources. Under this condition, tannase activity reached to 51.2 U/gds after 96 h of cultivation, which was 3.6 times as the conventional sterile fermentation. The results above revealed that under uncooked material fermentation for tannase production was a feasible method with high efficiency.