一株丁草胺降解菌的分离鉴定及其降解特性的研究

Isolation and Characteristics of a Butachlor Degradation Bacterium

利用富集培养技术从长期施用丁草胺的稻田土壤中分离得到能够降解丁草胺的细菌1株,标记为LYC-1。经形态特征、生理生化特征和16SrRNA序列分析,将该菌株鉴定为不动杆菌属(Acinetobacter sp.),菌株LYC-1的最适生长温度为30°C,最适pH值为7.5。当接种量为5%时,该菌株在含100mg/L的丁草胺无机盐基础培养液中培养7d后,可使丁草胺降解达80%以上。

One Butachlor-degrading bacterium named LYC-1 was isolated from a soil sample by enrichment culture. Combining morphologic characteristics and physiobiochemical characteristics with 16S rRNA sequence analysis, strain LYC-1 was identified as Acinetobacter sp.. The optimal temperature and pH value for growth of strain LYC-1 was 30℃ and pH 7.5, respectively. The strain could degrade butachlor more than 80% when it was cultured in 100 mg/L butachlor medium for 7 days.