摘要
报道了HPLC法测定氧氟沙星胶囊的含量.选ODS色谱柱,以维生素B6为内标物,乙腈水磷酸三乙胺(105∶386∶3∶6)为流动相.氧氟沙星对照品与内标物的浓度之比为横坐标,峰高比为纵坐标作图,线性范围为008~08mg·mL-1,相关系数r=09998,平均回收率为1001%,相对标准偏差RSD=017%.
A method has been establised for the quantitative determination of ofloxacinum in its capsules by HPLC.An ODS column and a mobile phase of acetonitrilewaterphosphoric acidtriethylamine (105∶386∶3∶6)were used and Vitamin B6 was selected as the internal standard. The linear relationship between the concentrate ratio of the standard ofloxacinum to internal standard and the ratio of their peak height was quite good. The correlation coefficient was 0999 8,the average recovery was 1001%,and RSD was 017%(n=5).
出处
《沈阳药科大学学报》
CAS
CSCD
1998年第3期202-203,共2页
Journal of Shenyang Pharmaceutical University
关键词
高效液相色谱法
氧氟沙星
胶囊
内标法
HPLC
ofloxacinum capsule
Vitamin B6
internal standardization