紫外分光光度法测定磷酸咯萘啶肠溶片的含量

DETERMINATION OF MALARIDINEIN ENTRIC-COATED TABLETS BY UV SPECTROPHOTOMETRY

目的研究磷酸咯萘啶在不同ｐＨ溶剂中紫外吸收特征变化，对药典采用的紫外分光光度法测定磷酸咯萘啶肠溶片含量的方法作了改进。方法以ｐＨ２的盐酸溶液作为溶剂，２７４ｎｍ处为测定波长。与用其他溶液作溶剂的方法相比，以优化测定条件。结果在０．５～２０ｍｇ／Ｌ浓度范围内，浓度与吸光度成线性相关，回归方程为Ａ＝０．００４５＋０．０５０４ｃ，ｒ＝０．９９９８（ｎ＝６），天内精密度ＲＳＤ为０．１５％，天间精密度ＲＳＤ为０．６０％，平均回收率为９６％以上。制剂辅料无干扰。结论本法与用水或ｐＨ７的磷酸缓冲液为溶剂的方法相比，具测定数据稳定，吸收系数大等优点。适用于磷酸咯萘啶肠溶片、注射剂及溶出度等测定。

おURPOSE To establish an ultraviolet spectrophotometry method for the determination of malaridine in enteric-coated tablets.METHODS The absorbence of malaridine was determined at 274nm by UV spectrophotometry, using hydrochloric acid (pH=2) as solvent.RESULTS According to the absorbence (A) and the concentration (c), a calibration graph was obtained. It was linear in the range of 0.5～20mg/L of mararidine. The equation was as follows: A=0.0045+0.0504c, r=0.9998(n=6). The average recovery was more than 96%. The RSD of within-day and day-to-day were 0.15%(n=5) and 0.65%(n=5), respectively.CONCLUSIONS The results show that the method we established has the advantage of fine precision, high accuracy, simple procedure and cheap reagents. There is no any interference from the enteric-coat and the reagent made no any pollution