枸杞叶黄酮纯化工艺研究

Study on purification technology of total flavonoids in wolfberry leaves

摘要：研究了用大孔树脂分离纯化枸杞叶总黄酮的工艺，采用AB-8、HP-20、D101三种大孔树脂对总黄酮极性分离纯化，结果表明D101大孔树脂对枸杞叶黄酮有较好的分离纯化性能，确定了纯化工艺中加水量为10倍，水洗剂用量5BV，乙醇洗脱浓度为30％和60％，洗脱体积为3～4BV，树脂连续使用3次后需再生，黄酮物质的高效液相色谱分析说明，醇提取物中92％是芦丁。

Purification of flavonoids of Lycium Chinese leaves using macroreticular adsorbents was studied Macroreticular adsorbents HP20, AB-8 and D101 were used to richen and purification flavonoids. Results indicated that the D101 showed a better comprehensive adsorption property. Some parameters were decided in purification technology that the amount of adding water 10 times, the volume of water 5 BV, the concentrtion of used EtOH 30% and 60%, and the adding volume 3-4 BV. The macroporous resin should be regenarated after using 3 times continually. Flavonoid compounds in leaves of Lycium bararum were analized by using HPLC, results showed that rutin was the main component, which has a high content of 92%.