固定化酶催化L-抗坏血酸苹果酸酯合成及其性能研究

Immobilized Malate Dehydrogenase-catalyzed Synthesis and Biological Activities of L-Ascorbyl Malate

采用固定化苹果酸脱氢酶为催化剂,在丙酮体系中合成L-抗坏血酸苹果酸酯,用高效液相色谱(HPLC)测定产物的含量。结果表明:添加固定化酶量4g/L,底物配位物质的量比1:3,温度55℃,L-抗坏血酸苹果酸酯的得率最高为69.30%。以大豆油为底物添加0.05%L-抗坏血酸苹果酸酯,存放至第7天,POV值仅为8.41meq/kg,L-抗坏血酸苹果酸酯具有明显的抗氧化活性。当添加0.18%L-抗坏血酸苹果酸酯时对大肠杆菌,金黄色葡萄球菌和枯草芽孢杆菌抑菌率分别为92.13%、90.84%和84.63%。说明L-抗坏血酸苹果酸酯是一种有潜力的抗氧化剂和抑菌剂。

Using immobilized malate dehydrogenase as the catalyst, L-ascorbyl malate was synthesized in an acetone system, and the product content was determined by HPLC. The results showed that when the catalyst amount was 4 g/L, the molar ratio of L-ascorbic acid to malic acid was 1：3, and the reaction temperature was 55℃, the highest L-ascorbyl malate yield of 69.30% could be obtained. In addition, when the addition of L-ascorbyl malate to soybean oil was 0.05%, the POV value on the 7th day was only 8.41 meq/kg, indicating that L-ascorbyl malate has significant antioxidant activity. When the addition of L-ascorbic malate was 0. 18%, its inhibitory rates against Escherichia coli, Staphyloccus aureus, and BaciUius subitills were 92.13%, 90.84% and 84.63%, respectively. It was concluded that L-ascorbyl malate is a potential antioxidant and bacteriostat.