HPLC法测定花生根茎叶中白藜芦醇的含量

HPLC Determination of Resveratrol in Roots,Stems and Leaves of Peanut

目的:测定花生根茎叶中白藜芦醇的含量。方法:建立花生根、茎、叶一步硅胶柱预处理、HPLC法测定其中白藜芦醇含量的方法。色谱柱为Hypersil ODS-2(4.6mm×250mm,5μm),流动相为甲醇-水(45:55,V/V),流速为0.8ml/min,柱温为35℃,紫外检测波长为306nm。线性范围:0.228～1.14μg(其相关系数R=0.9999),检测限4.3ng(3σ)。结果:测得山东、江苏和河北产花生根茎叶混合物中白藜芦醇的含量分别为903.69、116.21、88.69μg/g;山东产花生不同部位花生根、茎和叶中白藜芦醇的含量分别为223.08、1211.19、429.79μg/g;平均回收率为99.6%,RSD为3.09%(n=5)。结论:该方法简便、快速、准确。

Objective： To determine the contents of resveratrol in roots, stems and leaves of peanut. Methods： A HPLC determination combined with one-step pretreatment by silica gel column chromatography was established. The analysis was achieved by separation on an Hypersil ODS-2 column （4.6 mm×250mm,5μm） at 35 ℃ using a methanol-water （45：55, WV） mobile phase at a flow rate of 0.8 ml/min followed by UV detection at 306 nm. Results： The contents of resveratrol in the mixtures of roots, stems and leaves of peanuts from Shandong, Jiangsu and Hebei provinces were 903.69, 116.21 and 88.69 μg/g, respectively. The contents of resveratrol in roots, stems and leaves of peanut from Shandong province were 223.08, 1211.19 and 429.79 μg/g, respectively. The calibration curve was well linear over the concentration range from 0.228 to 1.14lag （R = 0.9999）, and the limit of detection was 4.3 ng （3σ ）. The average recovery was 99.6%with a RSD of 3.09% （n = 5）. Conclusions： This method is simple, fast and accurate.