摘要
以中熟桃‘京玉’和‘大久保’果实为材料,测定桃果实内果皮在细胞分裂和硬核期糖的含量,分析糖积累的变化情况;通过间苯三酚—HCl对‘大久保’桃果实内果皮进行染色,观察木质素沉积的进程;应用常规戊二醛—锇酸双固定法在透射电镜下观察内果皮细胞壁的发育进程。结果表明,桃果实内果皮在花后40d前,细胞不断分裂增生,初生细胞壁不断膨大,糖的积累快速增长,大约40d时葡萄糖和果糖的积累量达到最高;到40d后,糖含量及山梨醇代谢迅速下降。此时内果皮开始木质化,内部逐渐呈现红色,大约半个月后,整个内果皮都变红,木质素从内到外基本沉积完毕,说明木质素沉积发生在糖积累高峰之后,糖的大量积累为木质素前体的合成奠定了物质基础,糖积累量的多少直接影响内果皮发育的好坏。
The experiment was conducted with peach (Prunus persica L. )‘ Jingyu' and‘Okubo' fruitS. The sugar content was measured in peach endocarp during the coupe of the cell, division and pit hardening, analyzing the change of sugar accumulation, observing the development of endocarp cell wall through the transmission electron microscope (TEM) and detecting the progress of the its lignin deposition by staining with phloroglucinol -HCl reagent. The results showed the cell in the peach endocarp divided continuously, the primary cell wall expanding, the cell organ marginalizing gradually, which was the stage of sugar rapid accumulation, before 40 days after full blooming. The internal part began red after more than 40 days, the lignin deposition finished after 15 days or so, sugar accumulation and dynamic metabolism decreased rapidly, which showed lignin deposition occurred after the peak of sugar accumulation. Its content had an important effect on endoearp development.
出处
《园艺学报》
CAS
CSCD
北大核心
2009年第8期1113-1119,共7页
Acta Horticulturae Sinica
基金
国家自然科学基金项目(30872029)
北京市自然科学基金重点项目(6071001)
北京市自然科学基金项目(6092007)
北京市教委重点项目
北京市教委平台建设项目
北京市都市农业学科群项目(XK100190553)
北京市属高校人才强教深化计划项目(PHR20090516,PHR200906134)
北京农学院青年基金项目
关键词
桃
果实
内果皮
糖积累
木质素
peach
fruit
endocarp
sugar accumulation
lignin deposition