黄瓜嫁接苗和自根苗的蛋白质组学研究

Proteomic Study on Grafted and Non-grafted Cucumber(Cucumis sativus L.)

为了进一步探讨黄瓜嫁接苗比自根苗抗病抗逆性好、光合能力强的机理,在塑料大棚和高温消毒基质栽培条件下,用双向电泳(2-DE)技术和质谱(MS)技术研究了‘黑籽南瓜’与‘津优1号’黄瓜嫁接苗和‘津优1号’黄瓜自根苗叶片的蛋白质变化。嫁接后分别于苗期、开花期和结果期测定嫁接苗和自根苗叶片的蛋白质。结果表明,嫁接苗叶片中新产生了两类蛋白质:能提高抗病抗逆能力的R蛋白(RGC693蛋白)和促进萜烯类物质合成的鲨烯合酶,能促进叶绿体合成的辅酶和提高光能利用率的捕光叶绿素a/b结合蛋白。它们的出现可以说明嫁接黄瓜的抗病抗逆能力和光合能力优于自根苗的原因。

Study was conducted to explain the reason why grafted cucumber （ Cucumis sativus L. ） performs better than non-grafted one in the respect of resistance or tolerance to biotic or abiotic stresses and photo-synthesis. In a plastic chamber, grafted cucumber ＇ Jinyou 1 ＇ and non-grafted cucumber‘Jinyou 1 ＇ were planted in plastic pots filled with pasterised medium consisting of peat, vermiculite and moss. Proteins from the leaves of the two types of cucumber plants were analyzed in seedling stage, flowering stage and fruiting stage by two-dimensional electrophoresis （2-DE） and mass spectrum （MS）. In the leaves of the grafted‘Jinyou 1 ＇ , two types of proteins were found to be induced to higher degree of expression than in the nongrafted plants. The first type of proteins was R-protein （ RGC693 ） and squalene synthase related to resistance or tolerance to the stresses. The second was coenzyme which promotes chloroplast synthesize, and chlorophyll a-b binding protein which enhances photosynthesis. So the results obtained in this research could partly explain the mechanism of the better stress tolerance and higher photosynthesis in the grafted cucumber plant.