脉冲磁场对脑缺血再灌注大鼠胰岛素生长因子表达的影响

The effect of pulsed magnetic field on expression of IGF-1 in cerebral ischemia reperfusion injured rats

目的:观察脉冲磁场对脑缺血再灌注大鼠胰岛素生长因子(IGF-1)表达的影响。方法:SD大鼠24只,随机分成假手术组、模型组和脉冲磁场组,每组8只。采用线栓法制备大鼠局灶性脑缺血模型,假手术组仅做右侧颈外动脉和颈总动脉结扎,不做右侧大脑中动脉线栓栓塞。脉冲磁场组在造模结束后2h给脉冲磁场处理,磁场强度为0—0.01T,频率为50Hz,20min/次,1次/d,7d后处死大鼠,断头取脑,采用免疫组化方法检测IGF-1的表达。结果:模型组与假手术组相比,IGF-1的表达增多;脉冲磁场组与模型组相比,IGF-1的表达增多(P<0.05)。结论:脉冲磁场能促进脑缺血再灌注大鼠IGF-1的表达。

Objective：To examine the effect of pulsed magnetic field in inducing insulin-like growth factor-1（IGF-1） expression in focal cerebral isehemia reperfusion injured model. Method：Twenty-four SD rats were randomized into 3 groups, namely, sham-operation group, model group and pulsed magnetic field group with 8 in each group. Middle cerebral artery occlusion （MCAO） was employed to establish focal cerebral isehemia prefusion model in rats of model group and pulsed magnetic field group. In sham-operation group rats＇ middle cerebral artery were only ligated without occlusion. The treatment of pulse magnetic field was at 50Hz, 0.01T, 20min, once daily, for 7d. The expression of IGF-1 in ischemie brain tissue was detected with immunohistochemieal staining. Result：The expression of IGF-1 in ischemic brain tissue was the highest in pulsed magnetic field group. Conclusion：Pulsed magnetic field might improve the expression of IGF-1 in cerebral isehemia reperfusion injured rats.