病理性瘢痕组织中微血管密度的测定及与survivin mRNA的表达关系

Measurement of microvessel density （MVD）and its relationship with expression of survivin mRNA in pathologic scar

目的测定病理性瘢痕组织中CD105标记的微血管密度（microvesiel density，MVD）和survivin mRNA的表达，探讨病理性瘢痕组织中癌基因在血管形成中的作用。方法采用多相寡核苷酸探针原位杂交技术和免疫组织化学染色技术（SP法），分别检测survivin mRNA、CD105蛋白在40例病理性瘢痕、20例非病理性瘢痕和20例正常皮肤组织中的表达，并计数CD105标记的MVD。结果CD105-MVD、survivinmRNA在病理性瘢痕较非病理性瘢痕和正常皮肤的表达差异皆具有统计学意义（P〈0.05）。病理性瘢痕组中survivin mRNA表达与CD105-MVD表达有相关性（P〈0.05）。结论病理性瘢痕组织中，凋亡抑制基因survivin表达上调可能参与了其血管形成过程。

Objective To study the microvessel density （MVD） marked by CD105 and expression of survivin gene in pathologic scar, and to investigate the function of oncogenes in tumorigenic process and abnormal scarring. Methods In situ hybridization and immunohistochemical methods （SP technique） were used to detect survivin mRNA, CD105- MVD in 40 pathologic scar cases, 20 normotrophic scar ca ses, and 20 normal skin cases, respectively. The relationship between the expression of survivin mRNA and CD105- MVD in 40 pathologic scar cases were analyzed. Results Compared with normotrophic scar and normal skin cases, the expression of survivin mRNA, CD105- MVD were significant difference in pathologic scar cases （P〈0.05）. In pathologic scar tissue, the expression of survivin had significant correlation with MVD marked by CD105 （P〈0. 05）. Conclusion survivin is up regulated in pathologic scar, which may contribute to the vascular formation.