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维酶素中辅酶和VB_2的液相色谱分析

Determination of Coenzymes and VB_2 in WeiMeiSu By HPLC
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摘要 维酶素作为营养保健食品填加剂,对萎缩性胃炎和食道上皮细胞增生有显著疗效。本文采用高压液相色谱法对维酶素中维生素B_2和两种辅酶(FMN和FAD)进行了分析。结果VB_2平均含量为5.46mg/g,辅酶含量为1.74mg/g,回收率为86.6%~93.5%,精度为8.2%,该法可做为鉴定维酶素质量的分析方法。 Weimeisu is an additive of nutritious health food, which has significantly active effect on cure of wasting gastritis and accretion of epithelialcells of esophagus. The active constituents are three forms of riboflavin, that is free vitamin 62 (VB2) and two coenzymes, Flavin mononucleotide (FMN) and Flavin adenine dinucleotide (FAD).VB2 and the two coenzymes in Weimeisu were analyzed by HPLC. Products from Beijing ZhongFa Bioengineering Institute and other factories were analyzed, the average value of VB2 was 5. 46mg/g and coenzyme was 1. 74mg/g, the rate of recovery was 86. 6% - 93. 5%, and the accuracy of analysis was 8. 2%. The value of VB2 was in accordance with the results got through the method of pharmacopoeia. The method could be used to certifict the quality of Weimeisu.
出处 《现代仪器使用与维修》 1998年第4期17-19,共3页 Modern Instruments
关键词 维酶素 高压液相色谱 维生素B2 辅酶 Weimeisu HPLC VB2 Coenzyme
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  • 1陈文为,郭兆同,刘培楠.核黄菌(Eremothecium ashbyii)在几种谷物上产生核黄素的试验[J]营养学报,1956(01).

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