痛风泰颗粒剂对痛风兔IL—1β、IL-8的影响

The effect of Tongfentai granules to cytokine IL -1β and cytokine IL - 8 of acute gouty arthritis rabbit.

目的观察兔急性痛风性关节炎模型关节液中白细胞介素—1β（IL—1β）、白细胞介素-8（IL-8）水平的变化及痛风泰颗粒剂对其的干预作用。方法将32只新西兰大白兔随机分为中药组、戴芬组、模型组、正常组，按照Me Carty，DJ经典造模方法建立急性痛风性膝关节炎模型，分别予痛风泰颗粒剂、戴芬、0．9％氯化钠注射液灌胃，7d后应用酶联免疫法（ELISA）检测膝关节积液中IL—1β、IL-8水平。结果中药组、戴芬组和模型组与正常组比较，IL—1β、IL-8均高表达（P〈0．01），中药组、载芬组与模型组比较，差异亦有统计学意义（P〈0．01），而中药组与戴芬组比较，差异无统计学意义（P〉0．05）。结论IL—1β、IL-8是急性痛风性关节炎的炎性介质，而痛风泰颗粒剂能显著降低兔急性痛风性关节炎模型关节液中IL—1β、IL-8水平，对急性病风性关节炎有较好的干预效应，且与戴芬相当。

Objective To observe the model of acute gouty arthritis rabbit interleukin —1β （ IL —1β） and interleukin - 8＇s （ IL - 8 ） changements and the effect of Tongfengtai Granules（ a mixture of Chinese medicinal herbs）. Methods thirty -two rabbits were randomly divided into four groups. Three of the groups were made to be acute gouty arthritis models. Then the one was Ig with Tongfengtai Granules, the one was Ig with Delayed Release Cupsules Dielofenac Sodium, the last was Ig with Sodium Chloride. Then test the joint effusion IL —1β, IL - 8 level with the application of enzyme - linked immunosorbent assay test（ELISA） method after 7 days. Results Compared to the normal group, the cytokine IL—1β and IL- 8 in synovial fluid were both high in Chinese medicine group, western medicine group and Sodium Chloride group（P 〈 0. 01 ）. Compared to the Sodium Chloride group, IL —1β and IL - 8 were remarkably decreased in Chinese medicine group and western medicine group （P 〈 0.01 ）. Chinese medicine group was similar with western medicine group （ P 〉 0. 05 ）. Conclusion Tongfengtai Granules can significantly reduce the rabbit model of acute gouty arthritis IL —1β, IL - 8 level, and have a better therapeutic effect to acute gouty arthritis.