β-环糊精用于毛细管电泳拆分佐匹克隆对映体的研究

Chiral Separation of Zopiclone by Capillary Electrophoresis with β-Cyclodextrin as Chiral Selector

以β-环糊精(β-CD)为手性添加剂,用毛细管区带电泳法对手性药物佐匹克隆进行了拆分研究。考察了β-环糊精浓度、背景电解质组成、浓度及pH值、分离电压、温度等对对映体分离的影响。结果表明,以pH2.5的100 mmol/L三羟甲基氨基甲烷-磷酸溶液(含15 mmol/Lβ-环糊精)为运行缓冲液,分离电压28kV,毛细管温度16℃,检测波长303 nm,佐匹克隆对映体达到最佳分离,分离度为2.7,对映体迁移时间分别为8.4、8.9 min。该方法简单、快速、经济,可适用于佐匹克隆对映体的手性分离。

A new method was developed for the enantioseparation of zopiclone by high performance capillary zone electrophoresis （HPCE） using β-cyclodextrin （β-CD） as chiral selector. The effect of concentration of β-CD, pH, composition and concentration of background electrolyte, separation voltage and temperature on the separation efficiency was optimized. The result indicated that an best separation of zopiclone enantiomers was obtained by using 15 mmol/L β-CD in 100 mmoL/L Tris - H3PO4 as background electrolyte at pH 2.5 with a constant temperature of 16 ℃ and applied voltage of 28 kV. The detection wavelength was set at 303 nm. Under the optimal condition, zopiclone enantiomers were successfully separated in 9 rain with a resolution（Rs） of 2.7. The migration times of R-（ - ）-zopiclone and S-（ ＋ ）-zopiclone were 8.4 rain and 8.9 min, respectively. The established HPCE method is simple, fast and economic, and is suitable for the chiral separation of zopiclone enantiomers.