羟丙基壳聚糖包覆胰岛素微球的性能研究

Study on properties of insulin coated with hydroxypropyl chitosan microspheres

以壳聚糖和环氧丙烷为原料合成了羟丙基壳聚糖,并以此为壳材,以胰岛素为芯材,制备了平均粒径为13.34μm的微球,并考察了微球的各种性能。通过红外光谱表征了羟丙基壳聚糖和微球的化学结构;热重分析考察了羟丙基壳聚糖微球和包覆胰岛素后微球的热稳定性;采用光学显微镜和扫描电镜观察了不同液体中微球和干品微球的形貌;采用紫外分光光度计绘制胰岛素标准曲线和测定微球的包埋率;并对微球室温下、模拟胃液和模拟肠液中进行了微球稳定性考察。所制备的微球表面光滑、致密、平均粒径适中,且分布较窄,收率和包埋率较高,室温下稳定。根据稳定性考察得出,微球有缓释的功效。

Purpose：hydroxypropyl chitosan was synthesized from chitosan and propylene oxide, and use it as the shell material, insulin as the core, prepared for micmspheres, the average particle size was 13.34μm. Method： the chemical structure of hydroxypropyl chitosan and the microspheres was characterized by IR; thermal stability of hydroxypmpyl chitosan microspheres and coated insulin microspheres coated with it was inspected by TG; morphology, of different microsphere in liquids and dried micmspheres was observed using both optical microscopy and scanning electron microscopy; insulin standard curve and deter mination of encapsulation efficiency of microspheres was determined by UV; the stability of the microspheres at room temperature,in simulated gastric juice and in simulated intestinal fluid was inspected. Results： the microspheres are surface smooth, dense, with a proper average size and narrow distribution. The yield and encapsulation efficiency reached 93% and 61.3% respectively. The microsphere were stable at room temperature. Conclusion： the microspheres have the efficacy of slow-release according to the stability.