针刺对局灶性脑缺血再灌注大鼠神经细胞凋亡的影响

Effect of Acupuncture on Neuronal Apoptosis in Rats with Focal Cerebral Ischemia Reperfusion Injury

目的研究针刺对局灶性脑缺血再灌注大鼠神经细胞凋亡的影响。方法建立大鼠中动脉阻塞再灌注模型(MCAO),应用TUNEL法观察神经元凋亡,免疫组织化学法检测Caspase-3蛋白表达。结果脑缺血再灌注后应用针刺疗法能够降低大鼠神经细胞受损后出现的Caspase-3蛋白表达,并能减轻神经细胞凋亡。结论针刺疗法能通过有效的抑制脑缺血再灌注后大鼠神经细胞的凋亡起到脑保护作用。

Objective To observe the effect of acupuncture on neuronal apoptosis in cerebral ischenmiareperfusion injury rats. Methods The model of ischemia-reperfusion injury was established by transient middle cerebral artery occlusion （MCAO）. The immunohistochemical technique and TUNEL staining method were used to determine the expression of Caspase-3 and neuronal apoptosis, respectively. Results The expression of Caspase-3 was increased significantly after cerebral ischemia-reperfusion. Acupuncture could markedly inhibit the expression of Caspase-3 and neuronal apoptosis （ P 〈 0.01 ）. Conclusion Acupuncture has a protectetive effect on cerebral ischemia-reperfusion injury by reducing the expression of Caspase-3 and inhibiting neuronal apoptosis.