一种改良的猪重症急性胰腺炎模型的建立

Establishment of a Better Pig Model of Severe Acute Pancreatitis

目的建立一种新的适用于治疗重症急性胰腺炎(SAP)的猪模型,为SAP肠内给药提供实验和理论依据。方法8只太湖梅山猪随机分成SAP组和对照组,每组4只。两组均在开腹直视下置入鼻空肠管。SAP组通过胰管内注射5%牛黄胆酸钠建立SAP模型,对照组仅行胰管插管不注射任何药物。比较两组动物一般情况、血清淀粉酶、CT评分及胰腺病理形态学改变,验证鼻空肠管置入及SAP模型诱导是否成功。结果同对照组比较,SAP组血清淀粉酶的总体均数明显升高,差异有统计学意义(P<0.05);CT、病理评分均进一步证明了鼻空肠管置入及SAP模型诱导成功。结论在猪的SAP模型上成功置入鼻空肠营养管,为研究肠内给药在胰腺炎中的应用建立了一种简单、无创、可重复性的动物模型。

Objective To establish a new severe acute pancreatitis （SAP） pig model for evaluating enteral adminstration therapy. Methods Eight tai-lake plum-mountain pigs were randomized into the SAP group（n=4） and the control group（n=4）. In anaesthetised eight pigs were orthophorie inserted a nasojejunal catheter. In the SAP group the pancreatic duct was cannulated and taurocholic acid was infused to induce severe acute pancreatitis. Pigs in the control group were cannulated alone and seved as the controls. Temperature, bowel sounds, serum amylase, computed tomography（CT） scores and histologic examination were compared between the two groups, to confirm that whether pancreatitis model was established or catheter was inserted. Results Compared with the control group, SAP group serum amylase was obviously increased （P〈0.05）. Both CT and histologie examination demonstrated that severe acute panereatitis model was established and nasojejunal catheter was inserted successfully. Conclusion It is a simple, noninvasive, repeatable model of enteral nutrition and administration through intestinum in pancreatitis treatment.