盐豆木固氮根瘤内ATP酶的定位研究

CYTOCHEMICAL LOCALIZATION OF ATPase ACTIVITY IN THE ROOTNODULES FROM Halimodendron Halodendron (Poll) Voss

对盐豆木根瘤进行了ＡＴＰ酶的电镜细胞化学研究．结果表明，ＡＴＰ水解酶的磷酸铅沉淀分布于宿主细胞质、胞间隙间及拟菌体周膜和液泡膜上．在宿主细胞中，正在发育液泡的膜上有高的ＡＴＰ水解酶活性．质体中ＡＴＰ酶的磷酸铅沉淀也较多．拟菌体内ＡＴＰ水解酶活性随拟菌体发育阶段的不同而异，幼壮龄拟菌体内ＡＴＰ水解酶磷酸铅沉淀较少，而在衰老拟菌体内则较多．这种动态变化，可能与拟菌体发育不同阶段的固氮活性有关．

The study on localization of ATPase in root nodules on Halimodendron Halodendron(Poll) Voss shows that the particles of lead phosphate precipitates resulting from the reaction of ATP hydrolytic enzyme distrbute in cytoplasm,peribacteroid membranes and tonoplast etc.of the host cells.ATPase plays an important role in the transportation and absorption of substances, The heavy lead phosphate deposits indicating a high activity of ATPase were obviously localized at tonoplast of developing vocuoles.Possibly,this results indicat that ATPase is related with the physiological function of the host cells at different development stages.