二色桌片参酶解液的抗胃癌细胞MGc80-3作用

The Effects of Enzymedigested Solution from Mensamariaintercedens (Lampart) on Gastric CancerCell MGc803 in Culture

用二色桌片参［Ｍｅｎｓａｍａｒｉａｉｎｔｅｒｃｅｄｅｎｓ（Ｌａｍｐａｒｔ）］酶解液处理培养的人胃腺癌细胞ＭＧｃ８０－３后有如下效应：对其生长的抑制率可达３２．７５％，经处理后细胞铺展更好，透光性较好，细胞表面较光滑，微绒毛少，核膜规则而光滑，核仁明显．在软琼脂中的集落形成率由０．０１６７％下降为０．００４５％．Ｃｏｎ－Ａ介导的细胞凝集率在１００μｇ／ｍＬ浓度中下降４０．２个百分点，在２００μｇ／ｍＬ中下降３５．４个百分点；对裸小鼠致瘤的瘤重下降３３．０７％．结果表明二色桌片参酶解液对该胃癌细胞的生长有一定的抑制作用，对其恶性表型有一定的逆转作用．

The purpose of this work is to study the anticancer effect of enzymedigested solution from sea cucumberMensamaria intercedens (Lampart) . The gastric cell line MGc803(Human Gastric Gland Carcinoma) was selected. Several data were detected and proved that this sea cucumber enzymedigested solution had effects on this cancer cell line. Results were shown as below: After treated, the growth inhibition percentage is 32.75% to this cell line. The cells have better transparency, spread well, and have fewer microvillies, obvious nucleoli. The nucleus has regular appearance with smoother membrane. The colony forming percentage in soft agar is decreased from 0.0167% to 0.0045%. The percentage of Con Amediated agglutination is decreased by 40.2% in 100μg/mL Con A group and 35.4% in 200 μg/mL Con A group. Tumor weight caused in the nude mice is decreased by 33.07%. The enzymedigested solution from Mensamaria intercedens (Lampart) has the effect of inhibiting the cancerous phenotype and can somewhat induce the differentiation of this cancer cell line, but whether it can affect other cancer cell lines and what is the mechanism should be further studied. 