用锌离子从人尿中分离尿激酶

Recovery of Urokinase from Human Urine by Precipitation with Zinc Ion

用低浓度锌离子从人尿中沉淀尿激酶（ＵＫ），锌离子浓度为３ｍｍｏｌ／Ｌ时，沉淀回收率为８７％～９３％，用ｐＨ１０～１０．５的２０ｇ／Ｌ硫酸铵按１∶４（ｇ∶ｍｌ）溶解ＵＫ沉淀物，ＵＫ溶解得率为９７％。溶解液用７０％饱和度的硫酸铵盐析，ＵＫ盐析收率为９１％，粗品纯度为４．０８×１０５ＩＵ／ｇ，固体效价为３．６×１０４ＩＵ／ｇ，总收率在７７％～８２％之间。每吨尿（５．００×１０６ＩＵ计）可得ＵＫ粗品（３．８～４．１）×１０６ＩＵ。该粗品可用离子交换层析或亲和层析进一步纯化。

Urokinase can be separated from human urine by Zn2+ precipition. The optimal Zn2+ concentration was found to be 3.0mmol/L by which 1.5g wet precipitation was obtained from a litter of fresh human urine with a yield of 87%￣92%. The precipitation can be dissolved with 20g/L(NH4)2SO4+ammonia (pH 10.0￣10.5). The resulting urokinase solution was salted out by (NH4)2SO4 Solution of 70% saturation. The urokinase yield is 91% with purity of 4.08×105 IU/g protein and the solid activity was 3.6×104 IU/g. (3.8￣4.1)×106 IU crude urokinase cound be obtained from 1 ton fresh human urine. The overall yield was 77%￣81%.