不同品系及不同性别淡色库蚊非特异性酯酶活性比较

Comparison of non-specific esterase activity in different strains and gender of Culex pipiens pallens

目的探讨不同品系及不同性别淡色库蚊非特异性酯酶(NSE)活性的差异,为应用生物化学方法检测蚊虫抗药性提供依据。方法以β-乙酸萘酯为底物,坚固蓝B盐为显色剂,测定单个蚊虫NSE活性。结果抗敌敌畏(Rd)和抗残杀威(Rp)品系淡色库蚊NSE活性水平明显高于敏感(S)品系,抗氯氰菊酯(Rc)品系淡色库蚊NSE活性水平与S品系相近。S、Rd、Rp、Rc4种品系淡色库蚊3日龄雌雄成虫NSE活性均为雌性大于雄性。结论测定NSE活性可判断蚊虫抗药性状况,不同性别蚊虫应分别制定判断标准。

To explore the diversity of non-specific esterase activity in different strains of adult male and female Culex pipiens pallens,the NSE activity of single mosquito was determined by using the β-naphthalene acetic acid ester （β-NA） as the substrate and solid blue B salt （fast blue B salt） as the developer. The results showed the NSE activities of anti-DDVP （Rd） and anti-Propoxur （Rp） strains of Culex pipiens pallens were significantly higher than that of sensitive （S） strains,and the NSE activity of anti-Cypermethrin （Rc） strains of Culex pipiens pallens was similar to that of S strain. As for the S,Rd,Rp and Rc strains of 3-day-old adult male and female Culex pipiens pallens,the NSE activity of female mosquito was higher than that of male mosquito.