乙肝病毒感染者血清3种细胞因子水平及其临床意义

Clinical significance of the serum IL-6,IL-8 and TNF-α level in patients with hepatitis B

目的观察3种乙型肝炎(下称乙肝)病毒(HBV)感染者血清IL-6、IL-8及肿瘤坏死因子α(TNF-α)水平及其临床意义。方法采用ELISA法检测HBV感染者与健康对照组血清IL-6、IL-8及TNF-α的水平。结果慢性HBV携带者和慢性乙肝患者组血清IL-6、IL-8和TNF-α含量均高于健康对照组,差异有统计学意义(P<0.01),慢性HBV携带者和慢性乙肝患者血清IL-6、IL-8含量高于非活动乙肝表面抗原(HBsAg)携带者,差异有统计学意义(P<0.01),非活动HBsAg携带者与健康对照组比较差异无统计学意义(P>0.05)。结论血清IL-6、IL-8和TNF-α含量变化与肝炎病变程度及病毒复制状况密切相关,其在血清中的水平可反映肝细胞损伤程度及病毒复制状况,动态监测其含量对临床病情评估及预后判断具有重要参考价值。

Objective To investigate the clinical significance of IL-6, IL-8 and TNF -a in HBV infected patients. Methods Using ELISA to test the abundance of IL-6, IL-8 and TNF -α in serums from normal control,chronic HBV carrier,chronic hepatitis B and inactive HBsAg carrier groups. Results IL-6,IL-8 and TNF-α protein levels were higher in chronic HBV carrier and chronic hepatitis B groups than that of normal control and inactive HBsAg carrier groups （P〈0. 01 ）. IL-6, IL-8 protein levels were higher in chronic HBV carrier and chronic hepatitis B groups than that of inactive HBsAg carrier groups （P〈0.01）. Moreover, there were no statistical differences of IL6, IL8 and TNF-α abundance in serums between normal control and inactive HBsAg carrier groups （P〉0. 05）. Conclusion The increased levels of IL6,IL8 and TNF alpha in serum might be associate with HBV duplication and hepatocellular damage,and it is of very importance to detect and movnifor the effects of the amount to the disease.