摘要
目的:建立尿中N-甲基甲酰胺(NMF)和N,N-二甲基甲酰胺(DMF)同时测定的毛细管气相色谱法。方法:采用均匀试验设计法优化尿样的前处理方法,HP-FFAP毛细管色谱柱分离,火焰离子化检测器检测,保留时间定性,内标法定量。结果:NMF、DMF浓度在0.01-8.00μg/μl范围内线性关系良好(r≥0.999);NMF和DMF的检出限分别为5.0×10^-4μg和4.0×10^-4μg;NMF和DMF的加标回收率分别为94.6%-105.0%和98.0%-108.8%。尿样在4℃冰箱中至少可保存14 d。结论:该方法准确、灵敏,检出限低,适用于尿中N-甲基甲酰胺和N,N-二甲基甲酰胺的同时测定。
Objective: To develop the capillary gas chromatographic analytical method for simultaneous determination of N-methylformamide(NMF) and N,N-dimethylformamide(DMF) in urine.Methods:Optimization on the sample pretreatment technique with uniform design was investigated.The urine samples were analyzed by capillary gas chromatography-flame ionization detector(GC-FID).Retention time was used for qualitative analysis and internal standard method for quantitative analysis.Results:Calibration of both NMF and DMF were linear in the range of 0.01~8.00 μg/μl(r≥0.999).The detection limit was 5.0×10^-4 μg and 4.0×10^-4 μg for NMF and DMF,respectively.The rates of recovery were 94.6%105.0% for NMF and 98.0%108.8% for DMF.The urine samples could be reserved at 4℃ for at least 14 days.Conclusion:The developed method with good accuracy and precision achieves lower limit of detection.The assay is suitable to determine NMF and DMF in urine simultaneously.
出处
《中国卫生检验杂志》
CAS
2009年第8期1701-1703,1706,共4页
Chinese Journal of Health Laboratory Technology
基金
环保公益性行业科研专项(200809103)