宁波地区2008年麻疹病毒分离株H和N基因特征分析

Genetic characterization of H and N gene from wild-type measles viruses isolated in Ningbo in 2008

目的：分析宁波地区2008年麻疹病毒H和N基因的序列特征。方法：采用RT-PCR扩增2008年宁波地区麻疹分离株血凝素蛋白（H）和核蛋白（N）C末端456 bp核苷酸并进行序列测定,与GenBank中麻疹病毒各基因型参考株比较并分析毒株变异情况。结果：共分离到11株麻疹病毒,均为H1a基因亚型。与H1a参考株China93-2的H和N基因的氨基酸同源性分别为98.5%-98.7%和96.1%-98.7%;与中国疫苗株S191的H和N基因的氨基酸同源性分别为95.0%-95.5%和86.8%-89.5%。结论：宁波地区2008年流行的麻疹病毒均为H1a基因亚型,与中国疫苗株S191在基因特征上存在明显差异。

Objective： To investigate the genetic characterizations of H and N gene of measles viruses currently prevalent in Ningbo city.Methods：Measles viruses were isolated from throat swab specimens collected from suspected measles cases and the H and 456 bp fragments of C terminus of nucleoprotein（N） gene were amplified by RT-PCR.Sequence analysis was conducted of all 11 virus strains while compared with other measles virus strain published in GenBank.Results：All of the 11 measles isolates belonged to genotype H1a.The homogeneities of amino acids of H and N gene were respectively 98.5%～98.7% and 96.1%～98.7% while compared to the representative H1a genotype strain China93-2,the datas were 95.0%～95.5% and 86.8%～89.5% while compared to Shanghai 191.Conclusion：Genotype H1a measles virus had been proven to have circulated in Ningbo,China in 2008.There are obvious differences on genetic characteristics between the isolates and Shanghai 191.