摘要
目的建立测定人血浆中磷酸西他列汀含量的高效液相色谱法。方法采用Symmetrix ODS-H为分析柱，甲醇-0．1％高氯酸水溶液（40：60）为流动相；流速为1．0mL·min^-1，检测波长为268nm，采用外标法定量测定。结果进样浓度在5～500μg·mL^-1范围内与峰面积呈良好线性关系，r=0．9993，方法回收率为96．1％-100．9％。最低检测限为2μg·mL^-1。日间、日内精密度均小于4％。结论此方法操作简便、灵敏，重复性好，适用于人血浆中磷酸西他列汀含量的测定。
Objective To establish RP-HPLC method for determination of sitagliptin phosphate in human plasma. Methods A Symmetrix ODS-H column was used with methanol-0.1% perchloric acid solution （40 ： 60） as mobile phase. The flow rate was 1.0mL·min^-1 and UV detection wavelength was 268nm. Results The linear range. 5-500μg ·mL^-1 , r=0. 9993. Limit of detection （LOD） 2μg·mL^-1. The relative standard deviations of inter day and intra-day were less than 4% （n= 5）. Conclusions This method is simple, sensitive and reproducible. It is suitable for determination of sitagliptin phosphate in human plasma.
出处
《中国药事》
CAS
2009年第8期758-760,共3页
Chinese Pharmaceutical Affairs
