青蒿油多相脂质体中维A酸的薄层鉴别及含量测定

TLC identification and content determination of tretinoin in Artemisia oil polyphase liposome

目的:建立青蒿油多相脂质体中维A酸的鉴别及含量测定方法。方法:采用薄层色谱法对维A酸进行定性鉴别,并采用高效液相色谱法同时测定维A酸及其降解产物异维A酸的含量。色谱柱:Dikma Kromasil C18柱(250mm×4.6mm,5μm);流动相:乙腈-0.1%磷酸溶液(88∶12);检测波长:355nm;柱温:30℃;流速:1.0mL·min-1;进样量:10μL。结果:在薄层层析色谱中能检出维A酸,维A酸在0.1016～1.016μg范围内具有良好的线性关系(r=0.9999);平均回收率为99.4%,RSD为0.35%。结论:该方法简便、快速、准确,具有良好的重复性和回收率,可作为该制剂中维A酸的质量控制方法。

Objective：To establish the method for the identification and content determination of tretinoin in Artemisia oil polyphase liposome.Methods：Tretinoin in Artemisia oil polyphase liposome was identified by TLC,and the content of tretinoin and isotretinoin were detected by HPLC simultaneously.The separation was carried out on a Dikma Kromasil C18 column（250 mm×4.6mm,5μm）.The mobile phase was acetonitrile-0.1% phosphoric acid solution（88∶12）,The detective wavelength was set at 355 nm,The column temperature was 30℃,The flow rate was 1.0 mL·min-1and the sample size was 10 μL.Results：Tretinoin could be detected by TLC,The calibration curve showed good linearity over the range of 0.1016～1.016μg（r=0.9999）.The average recovery was 99.4%with RSD 0.35%.Conclusion：This method is simple,rapid,accurate and with good repeatability and recovery,it can be used as the quality control method for this preparation.