巫山淫羊藿中朝藿定C的含量测定及特征图谱初步研究

HPLC determination of epimedin C and primary investigation on specific chromatogram of Epimedium wushanense T. S. Ying

目的:建立反相高效液相色谱法测定巫山淫羊藿中朝藿定C含量和特征图谱的研究。方法:采用SUNFIRETM C18柱(250mm×4.6mm,5μm)色谱柱,以25%乙腈的水溶液为流动相进行洗脱,流速lmL·min-1,检测波长270nm,柱温30℃;通过对24份巫山淫羊藿药材HPLC图谱的检测,建立了巫山淫羊藿的HPLC特征图谱共有模式,并对11批药材进行了相似度比较。结果:巫山淫羊藿中各种成分均达到基线分离,朝藿定C的线性范围为0.071～2.82μg(r=0.9999),平均回收率(n=5)为101.3%(RSD=0.73%);得到的色谱图可为巫山淫羊藿药材专属性的特征图谱研究提供参考,为巫山淫羊藿药材的鉴别和质量控制提供了实验依据。结论:本法快速、准确、可靠,可用于巫山淫羊藿药材的质量评价。

Objective：To establish a quantitative evaluation method of epimedin C by RP-HPLC.Methods：Epimeidin C was base-isolated on the column of SUNFIRETM C18（250 mm×4.6 mm,5 μm）;The mobile phase was 25% acetonitrile aqueous solution at a flow rate of 1 mL·min-1;The detection wavelength was set at 270 nm and column temperature at 30 ℃.Results：The compounds were base-isolated.The linear range of epimedin C was 0.071-2.82 μg（r =0.9999）;The average recovery（n=5） was 101.3%（RSD=0.73%）.The mutual mode of HPLC specific chromatograms was set up and the similar degrees to the crude drugs were compared.Conclusion：The method is rapid and reliable,and it was capable of effectively controlling the quality of Epimedium wushanense T. S. Ying.