牡丹籽油超声辅助提取工艺优化及其GC-MS分析

Optimization of ultrasonic-assisted extraction of oil from Paeonia suffruticosa Andr.seeds and its GC-MS analysis

目的:以牡丹籽为原料,利用超声辅助提取牡丹籽油。方法:通过单因素实验和正交设计实验考察了液料比、超声波功率、处理时间、温度和提取次数等因素对牡丹籽出油率的影响,确定了超声辅助提取牡丹籽油的较优工艺条件,并用GC-MS对牡丹籽油组分进行了分析。结果:以沸程60～90℃的石油醚作为溶剂提取牡丹籽油的较优工艺参数为:液料比8mL/g,超声波功率为350W、提取温度40℃,提取时间为30min,提取次数为3次。在该工艺条件下,牡丹籽出油率为24·89%。结论:该方法工艺简便合理,提取率较高,所得牡丹籽油中富含不饱和脂肪酸,其中亚油酸和亚麻酸的含量分别为22·78%和64·14%。

Objective： The ultrasonic-assisted extraction technology was used to extract the seeds of Paeonia suffruticosa Andr.Method ：The single factor and orthogonal design experiments were adopted to study the effects of such factors liquid-solid ratio, ultrasonic power, extraction temperature and time on oil extraction rate.The seeds oil was analyzed by GC-MS to determine constituents.Result：The optimal technological condition of oil extraction was determined and the parameters of oil extraction using petroleum ether with boiling rang of 60-90℃ as the solvent should be as follows： the liquid-solid ratio was 8mL/g, the ultrasonic power was 350W, the extraction temperature was 40℃,the extraction was 30min, and under such condition, the total oil extraction rate after three times extractions is 24.89%.Conclusion：Based on the results of GC-MS analysis, unsaturated fatty acids such as linoleic acid and linolenic acid abound in Paeonia suffruticosa Andr.seeds,the content of linoleic acid and linolenic acid were 22.78% and 64.14%. respectively.