摘要
目的:从DNA水平上分析野生型明党参和栽培得到的明党参植株之间的遗传变异。方法:通过RAPD分子标记方法,筛选了80个随机引物,其中有52条随机引物能够扩增出条带,从中选取7个多态性强、重复性好的引物进行扩增。结果:实验共检测到31个位点,13个多态性位点,多态位点比率为41.9%,平均每个引物扩增4~5个条带,多态性条带2个。聚类分析显示野生明党参和栽培明党参之间遗传间距达0.277。结论:研究结果表明RAPD分子标记方法可以鉴定野生和栽培明党参,结果也表明明党参在栽培过程中出现了较强的遗传变异。
Objiective: To assess the genetic variation between the wild Radix Changii and the planted. Methods Random amplified polymorphic DNA (RAPD) analysis was used as a tool. Seven random primers of high reproducible and stable quality were sucessfully used to analyze genomic DNAfrom wild Radix Changii and the planted from 80 primers. Results There were 13 polymorphie fragments of 31 total fragments. The clustal analysis results were indicated that the average genetic distance between the wild Radix Changii and the planted was 0. 277. Conclutions The result show that RAPD technology can be used for genetic diversity researches in species of Radix Changii. The result also show that there are much Genetic Variation happened during planting of Radix Changii.
出处
《成都中医药大学学报》
2009年第2期80-82,共3页
Journal of Chengdu University of Traditional Chinese Medicine
基金
江苏省中医药管理局(编号:H05167)