滋养层细胞囊泡与奶牛低品质胚胎共移植对受胎率的影响

Effects of Trophoblastic Vesicles and Co-transplantation of Low-quality Bovine Embryos on the Pregnancy Rate

将奶牛低品质胚胎与囊胚滋养层细胞囊泡（trophoblastic vesicles,TRV）共移植,TRV来源于体外受精培养7 d、体内培养7 d囊胚的囊胚滋养层细胞。胚胎移植受体牛超声波妊娠诊断共进行2次,即发情的第26~43天和第38~73天。第1次妊娠诊断时试验组（与TRV共移植组）妊娠率（66.7%,16/24）显著高于对照组（34.5%,10/29）（P〈0.05）。在第1次和第2次妊娠诊断期间试验组有3头牛的胚胎死亡。试验组和对照组的分娩率分别为41.7%（10/24）和27.6%（8/29）,两者间无显著差异。两组间受体牛的妊娠期和后代出生重相似。

Low-quality bovine embryos were co-transplanted with trophoblastic vesicles （TRV）. These TRV were prepared by dissection of conceptus that were collected by uterine flushing after culture for seven days in the uterus following transfer of embryos derived by in vitro fertilization （IVF）. Pregnancy diagnoses were performed twice, in between day 26th and 43rd , between day 38th and 73ra post-estrus by ultrasonography. The pregnancy rate was significantly increased at first pregnancy diag-noses when low-quality bovine embryos were co-transplanted with TRV （66. 70/00, 16/24） compared with the control group （34. 5%, 10/29） （P〈0. 05）. The final pregnancy rate according to delivered offspring was 41.7%（10/24） and 27.6% （8/29）, respectively. There were no statistically significant differences between the two groups. The gestation lengths and birth weight of the offspring were almost the same for the co-transplantation and control group.