鸡新城疫和产蛋下降综合征二联灭活氢氧化铝胶疫苗免疫研究

Study on An Aluminium Hydroxide Gel Adsorbed Inactivated Newcastle Disease Virus and Egg Drop Syndrome Vaccine

使用鸡新城疫病毒LaSota株和产蛋下降综合征病毒分离株HE03株制备鸡新城疫和产蛋下降综合征二联灭活氢氧化铝胶疫苗。把40只16周龄SPF蛋鸡分成A、B组,分别是30只、10只。A组接种0.5 mL鸡新城疫和产蛋下降综合征二联灭活氢氧化铝胶疫苗,B组作为对照组,每隔14 d采集血清样本直到接种后第6周。至22周龄时,A组再次分成A1和A2两组。A2组与B组的鸡接受强致病性的产蛋下降综合征病毒攻击。结果表明,A组在不同时间采集的血清抗鸡产蛋下降综合征病毒的最高的抗体出现在接种后的第4周,抗鸡产蛋下降综合征病毒的HI抗体滴度从1∶128到1∶512不等,几何平均滴度(GMT)为1∶111;第4周时,抗鸡新城疫病毒的HI抗体滴度GMT为1∶134。该疫苗能保护鸡抵抗强致病性的产蛋下降综合征病毒和鸡新城疫病毒的感染,B组在攻毒感染后产蛋率显著下降,出现畸形蛋、软壳蛋和无壳蛋。

An inactivated vaccine was prepared from Newcastle disease virus LaSota strain and local isolates of egg drop syndrome （EDS） virus HE03 strain using aluminum hydroxide as adjuvant. A total of 40 SPF layers at the age of 16 weeks were inoculated and divided into two groups, A and B having 30 and 10 chicks, respectively. The chicks of group A were vaccinated with 0.5 mL aluminium hydroxide gel adsorbed ND-EDS vaccine. The chicks of group B were kept as unvaccinated control. The serum samples were collected at a 14 day interval till 6 weeks post vaccination. At the age of 22 weeks, group A was again divided into two groups A1 and A2; chicks of group A2 and group B were challenged with a virulent EDS virus HE03 strain. The results revealed that chicks of group A had HI antibody titer against ND virus ranging from 1 ： 128 to 1 ： 512 at different times titer against EDS virus ranging from 1 ： 128 to 1 ：512 at different times of sample collection with a geometric mean titer 1 ： 134; highest antibody response against EDS virus appeared on 4th week post vaccination with 1 = 111. Results also revealed that the vaccination protected the chicks against challenge with the virulent EDS virus and ND virus. Where there was a significant drop in egg production in unvaccinated challenged chicks group B, the egg quality was also deteriorated as miss-shapened, sofvshelled and shell-less eggs were laid in unvaccinated layers.