野西瓜水溶性生物碱抑制HepG-2增殖作用

Study on inhibition of water-soluble alkaloids in capparis spinosa in proliferation of human HepG-2 cell

研究野西瓜生物碱的抗肿瘤作用.采用MTT实验观察野西瓜水溶性生物碱的体外抗肿瘤作用,采用流式细胞仪测定野西瓜水溶性生物碱对肿瘤细胞凋亡及细胞周期的影响.10、50、100、150、200、300μg/mL的野西瓜水溶性生物碱作用于HepG-2细胞72 h后均可不同程度的抑制HepG-2细胞的增殖,其中野西瓜水溶性生物碱IC50为171.5μg/mL;作用48 h后出现凋亡形态;180μg/mL、360μg/mL的野西瓜水溶性生物碱作用48 h时出现细胞凋亡;周期分布发生改变.结果表明,野西瓜水溶性生物碱具有抗肿瘤作用.

To study the anti-neoplastic effect of alkaloids. MTT are used to study anti-neo- plastic action of oil in vitro. The effect of water-soluble alkaloids on cell apoptosis and different stages of cell cycles in human HepG - 2 cells is studied by flow eytometry. The proliferation of HepG - 2 cells is inhibited after treated with 10,50,100,150,200 and 300 μg/mL the water-soluble alkaloids in capparis spinosa for 72 h and the IC50 is 171.5 μg/mL. The apoptotic cells are detected ＂after treating with 180 and 360 μg/mL of the water-soluble alkaloids in capparis spinosa for 48 h. Cell cycle is also analyzed, with results showing that water-soluble alkaloids in capparis spinos leads to obstructing of S and G2 stage. Water-soluble alkaloids have significant anti-tumour effect.