大连滑刃线虫新种(线虫门,滑刃科)记述(英文)

APHELENCHOIDES DALIANENSIS SP. NOV. (NEMATODA,APHELENCHOIDIDAE) FROM PINUS THUNBERGII FROM CHINA

新种大连滑刃线虫Aphelenchoides dalianensis sp.nov.采自中国辽宁省大连市老铁山的枯死黑松。新种的鉴别特征为:体较短(雌虫:571.5～658.0μm;雄虫:436.8～520.0μm),口针纤细(雌虫:10.0～12.7μm;雄虫:9.2～11.8μm)具有基部球,侧线4条。雌虫阴门位于虫体60%～75%处,尾型特殊,具蜗牛触角状分叉的尾尖突;雄虫尾部向腹面弯曲成拐杖形,有1简单尾尖突,交合刺小(10.0～12.9μm),乳突3对,无交合伞。新种的近似种是大核滑刃线虫A.macronucleatus,主要区别在于大核滑刃线虫的雌虫仅具一简单尾尖突,雄虫加热杀死后呈"L"形,而非新种的"J"形。应用限制性酶切图谱(PCR-ITS-RFLP)的方法以及DNA测序为新种提供了分子生物学的证据。词源:新种是以标本采集地所在的城市大连命名。

A new species of nematode, Aphelenchoides dalianensis sp.nov. was extracted grom a wilt black pine （Pinus thunbergii） from Laotieshan （38.8°N, 121.2°E）, Dalian City, China. A. dalianensis was characterized by its length （females： 571.5-658.0 μm; males： 436.8-520.0 μm）; slender stylets （females： 10.0-12.7 μm; males： 9.2-11.8 μm） with basal knobs; 4 lateral lines. Female vulva post median, at 60%-75% of the body length; female tail with a special mucronate structure which had a divarication, forming an antennae-like terminus; male tail strongly hooked ventraUy to from the characteristic ＇ walking-salking form, tapering to a single terminus; small spicule （ 10.0-12.9μm） ; 3 pairs of caudal papillae and bursa absent. A. macronudeatus resembles the new species, espedally in male spicule, but differs from female tail temainus and the shape of male after heat-kiUed. The PCR-ITS-RFLP pattern and its rDNA sequence also provided further evidence that this isolate is 1 new species. Aphelenchoides dalianensis sp. nov. was named by the name of city, Dalian, where it was found.