不同光谱能量分布对文心兰组织培养的影响

Effects of different spectral energy distribution on tissue culture of Oncidium in vitro

采用发光二极管(light-emitting diode,LED)调制获取不同光谱能量分布的光源,以荧光灯为对照,研究其对文心兰原球茎诱导、增殖及生根组培苗生长的影响。结果表明:红光有利于原球茎的诱导,并促使原球茎产生最高的增殖系数和碳水化合物含量以及最低的分化出芽率;蓝光使原球茎产生最高的蛋白质含量、酶系活力和分化出芽率;单一红光促进文心兰生根苗茎的伸长,但不利于叶片色素的形成;单一蓝光抑制生根苗茎的伸长,促进叶片蛋白质和色素的合成;红蓝光谱分布处理的生根苗的生长量、干重、能效和酶系活力指标均高于荧光灯对照,色素含量与荧光灯对照无显著差异;红光LED促进文心兰原球茎的诱导,蓝光LED促进分化,红蓝光谱构成的LED光源更有利于生根组培苗的正常生长,是替代荧光灯的理想光源。

The light sources with different spectral energy distribution were generated by light-emitting diode （LED）, and then under these light sources, the protocorm-like body （PLB） induction, proliferation and growth of plantlets in rooting stage of Oncidium （Onc. ＇ Sweets Sugar＇ ） in vitro were investigated, while the fluorescent lamp was used as control. The results indicated that the PLB induction from shoot apex tissues was promoted obviously under the red LED with the highest proliferation rate, the most content of carbohydrate and the lowest differentiation rate, while the most content of protein content, enzyme activity and the highest differentiation rate were observed under blue LED. Under red LED, the elongation of plantlets of Oncidium in rooting stage was the highest, and the pigments were the lowest, whereas, the elongation was the least, and the pigments and protein were the highest under blue LED. In comparison with fluorescent light, the growth amount, dry mass, energy efficiency and enzyme activity of plantlets in rooting stage were the highest under combination of red and blue LED, and no significant difference was observed on pigment in leaf. Though the red LED is effective for PLB induction, and blue LED is advantageous for differentiation from PLB, the spectral energy distribution with a combination of red and blue LED is more suitable for the growth of plantlets in rooting stage.