摘要
目的:观察慢性荨麻疹患者单个核细胞表面TLR2、TLR4表达及其与血清IL-5的相关性;初步探讨TLR2、TLR4在慢性荨麻疹发病机制中的作用,为临床治疗提供新思路。方法:采用流式细胞仪检测49例慢性荨麻疹患者外周血单个核细胞TLR2及TLR4受体表达情况;用双抗夹心ELISA方法检测患者血清中IL-5浓度,与30例同期正常对照。结果:慢性荨麻疹患者外周血单个核细胞TLR2表达均明显高于正常对照,而TLR4表达与正常对照无明显差异(P>0.01),血清中IL-5浓度明显高于正常对照,TLR2表达与血清IL-5浓度呈正相关,TLR4表达与血清IL-5浓度呈无相关。结论:研究提示TLR2可能在慢性荨麻疹的发病中起重要作用,其机制可能是通过上调TLR2表达促进IL-5分泌来介导变态反应。
Objective :To discuss the role of Toll- like receptor (TLR2)/TLR4 in chronic urticaria and to provide a new method to cure the chronic urticaria, the expression of TLR2 on peripheral blood rnononuclear cells and its correlation with concentration of IL -5 in the patients with the chronic urticaria were investigated. Methods:The expression of TLR2 /TLR4 mRNA on peripheral blood mononuclear cells in 49 cases of the chronic urticaria was examined by flow cytometry, the content of IL -5 in serum was examined by ELISA. Blood from thirty cases of healthy people was used as control group. Results:The expression of TLR 2 and the serum level of IL -5 of patients were significantly higher than those in control group (P 〈 0.01 ), and the expression of TLR2 was positively correlation with level of IL -5. Conclusion:TLR2 may play a critical role in the mechanism of the chronic urticaria, which may upregulate the secretion of inflammatory factors to induce onset of chronic urticaria.
出处
《岭南皮肤性病科杂志》
2009年第4期213-215,共3页
Southern China Journal of Dermato-Venereology
基金
广州市皮肤病防治所为本项目提供科研基金.