雷公藤多甙对变应性接触性皮炎患者外周血调节性T细胞的影响

Effects of triptergium glycosides on regulatory T cells in patients with allergic contact dermatitis

目的:通过检测变应性接触性皮炎(ACD)患者经雷公藤多甙(TG)治疗前后外周血CD+4、CD+25、CD-127调节性T细胞(Treg)占总T淋巴细胞的百分比的变化,初步探讨TG治疗该病的作用机制。方法:选取斑贴试验确证镍为主要过敏原的ACD患者,随机纳入TG试验组52例和外用润肤霜对照组30例,在治疗前和治疗2周后,分别采患者外周血,通过流式细胞仪检测患者的Treg的百分比,同时观察患者的临床疗效。结果:TG治疗组患者外周血Treg占总T细胞的百分比较治疗前明显升高(P<0.05),并且患者症状有效改善;对照组治疗前后各指标比较无统计学差异。结论:TG提高ACD患者外周血Treg的百分比,可能是其发挥治疗作用的机制之一。

Objective：To investigate the role of triptergium glycosides （TG） on CD4^＋ CD25^＋ CD127^- regulatory T cells （Tregs） in the peripheral blood of patients with allergic contact dermatitis （ACD）. Methods：A group of 82 patients with ACD was included in the study. The main allergen was identified as nickel and confirmed by patch test. Tregs in peripheral blood of patients were detected by three -color flow cytometry before and two weeks after teatment with TG （52 cases） or topical moisturizer only （30 cases） as controls. Results：After treated by TG, average percentile of Tregs was increased significantly from （2.18 ±0.56 ） % to （4.35 ± 0.39 ） % （ P 〈 0.05 ） in TG group. However, the increase was not seen in the control group with topical use of moisturizer. Conclusion ： Increase of average percentile of Tregs in peripheral blood by TG may contribute to its effect on ACD.