摘要
目的 建立尿液中吗啡类药物的固相萃取／LC—MS／MS方法。方法采用OASIS MCX3cc（60mg）固相萃取柱进行提取，应用LC—MS／MS方法进行检测，运用保留时间和MRM方式对尿液中吗啡类药物及其代谢物进行定性定量分析。结果磷酸盐缓冲液pH4．0时，海洛因、6-MAM、可待因、吗啡、M3G的固相萃取回收率分别达64．33％-70．21％，96．95％～117．57％，83．60％～123．63％，68．82％～91．03％，94．64％～107．33％；最低检测限（LOD）分别为5、10、5、5、2pg，线性范围0．005～10μg／mL；相关系数分别为0．9998、0．9958、0．9992、0．9994、0．9997。结论本文所建方法，适用于尿液中吗啡类药物的分析。
Objective To establish a solid phase extraction （SPE）/LC-MS/MS method for determination of morphines in human urine. Methods Morphines were extracted from human urine using OASIS MCX 3cc（60mg） extraction cartridges and detected by LC-MS/MS. Qualitative and quantitative analysis was obtained by retention time （Rt） and Multiple Reaction Monitoring （MRM） of LC-MS/MS. Results The recoveries for heroin, 6-MAM, codeine, morphine, M3G were 64.33% -70.21%, 96.95% - 117.57%, 83.60% - 123.63%, 68.82% -91.03%, 94.64% - 107.33% respectively, using phosphate buffer, pH4.0. Limits of Detection （LOD） were 5pg for M3G, 10pg for morphine, 5pg for codeine, 5pg for 6-MAM and 2pg for heroin. The linear range is 0. 005 - 10μg/mL. The correlation coefficient of heroin, 6-MAM, codeine, morphine, M3G were 0. 999 7,0. 999 4,0. 999 2,0. 995 8,0. 999 8 respectively. Conclusion The results of this study suggest that the method is simple, rapid and accurate for quantitative analysis of morphines and their metabolites in human urine.
出处
《中国法医学杂志》
CSCD
北大核心
2009年第4期242-245,共4页
Chinese Journal of Forensic Medicine
基金
国家科技支撑计划项目(2006BAK09B04)
