益肾胶囊对狼疮性肾炎大鼠的治疗作用

The therapeutic effect of Yishen Capsule on lupus nephritis rats

目的探讨益肾胶囊对狼疮性肾炎(lupus nephritis,LN)大鼠的治疗作用及其作用机制。方法观察益肾胶囊组大鼠肾功能、24h尿蛋白(PRO/24h)的变化,分别采用双抗体夹心酶联免疫吸附法及放射免疫法检测血清Th1/Th2细胞因子(IL-2、IL-10、TGF-β1、IL-6、TNF-α)水平的变化和光镜观察肾脏病理形态学的改变。结果经过6周的治疗,益肾胶囊组及强的松组大鼠肾功能、24h尿蛋白及血清IL-6、IL-10、TGF-β1、TNF-α水平与模型组比较均明显降低,IL-2明显升高(P<0.05或P<0.01),且益肾胶囊组大鼠肾功能、24h尿蛋白及血清IL-6、TGF-β1、TNF-α水平明显优于强的松组(P<0.05或P<0.01);益肾胶囊组及强的松组肾脏病理改变均有不同程度的改善,两组比较无明显差异。结论益肾胶囊可明显降低LN大鼠24h尿蛋白和改善肾功能,其作用机制可能与调节血清Th1/Th2细胞因子网络平衡及减轻肾脏病理损害有关。

Objective To investigate the therapeutic effect of Yishen Capsule on lupus nephritis （LN） in rats and its mechanism. Methods We observed the changes of renal function and proteinuria /24 h of LN rats in Yishen Capsule group. At the same time, Th1/Th2 cytokines （IL-2, IL-10, TGF-β, IL-6 and TNF-α） in blood serum were measured by double antibody sandwich-ELISA and radioimmunity, respectively. The pathological changes of the kidney were observed by light microscope. Results After six weeks＇ treatment, renal function, proteinuria/24 h as well as serum levels of IL-6, IL-10, TGF-β1 and TNF-α were decreased and IL-2 was increased significantly in Yishen Capsule group and prednisone group compared with those in the model group （P〈 0.05 or P 〈 0.01）. Renal function, proteinuria /24 h, serum levels of IL-6, TGF-β1 and TNF-α were better in Yishen Capsule group than in prednisone group （P 〈0.05 or P 〈0.01）; there was some degree of amelioration in renal pathological changes, but with no differences in both treatment groups. Conclusion Yishen Capsule can lower proteinuria/24h and improve the renal function of LN rats, the mechanism may be related to regulating the balance of Th1/Th2 cytokine network and alleviating the renal pathological lesion.