肺岩宁方对人肺癌细胞系增殖及肿瘤坏死因子α诱导的白细胞介素6和白细胞介素8产物的影响(英文)

Effects of Feiyanning Decoction on proliferation of lung adenocarcinoma cell line and their production of interleukin-6 and interleukin-8 induced by tumor necrosis factor-α

目的:探讨中药肺岩宁方含药血清对人肺癌细胞系A549细胞增殖及肿瘤坏死因子α(tumor necrosis factor-α,TNF-α)诱导的A549细胞分泌白细胞介素6(interleukin-6 ,IL-6)和IL-8的影响。方法:肺岩宁方含药血清培养A549细胞24、48和72 h后,采用四唑单钠盐法检测肺岩宁方含药血清对A549细胞增殖的影响;肺岩宁方含药血清培养A549细胞48 h后,酶联免疫吸附测定法检测肺岩宁方含药血清对TNF-α诱导的IL-6和IL-8分泌的影响,逆转录聚合酶链式反应检测IL-6和IL-8 mRNA表达水平的变化。结果:肺岩宁方含药血清对A549细胞增殖具有明显的抑制作用。1μg/L TNF-α可明显提高A549细胞上清液中IL-6和IL-8的分泌水平,肺岩宁方含药血清与TNF-α共同作用后,可抑制IL-6和IL-8的分泌(P<0 .01)及IL-6、IL-8 mRNA的表达(P<0 .01)。结论:肺岩宁方含药血清对TNFα诱导A549细胞分泌IL-6和IL-8具有明显的抑制作用,这为其临床治疗肺癌提供了一定的实验依据。

Objective： To study the effects of Feiyanning Decoction, a compound traditional Chinese herbal medicine, on proliferation of lung adenocarcinoma cell line A549 cells and their production of interleukin-6 （IL-6） and IL-8 induced by tumor necrosis factor-α （TNF-α）. Methods： A549 cells were incubated with rat serum containing Feiyanning Decoction at 15% for 24, 48 and 72 h respectively. The cell proliferation was examined by 2-（2-methoxy-4-nitrophenyl）-3-（4-nitrophenyl）-5- （2, 4-disulfophenyl）-2H-tetrazolium, monosodium salt assay （WST-8）. The production of IL-6 and IL-8 was tested by enzyme-linked immunosorbent assay after 48-hour treatment of reagents, and the expressions of IL-6 and IL-8 mRNAs were detected by reverse transcription-polymerase chain reaction. Results： Serum containing Feiyanning Decoction had obvious inhibitive functions in A549 cell proliferation after 48- and 72-treatment. TNF-α （1μg/L） strongly induced the production of IL-6 and IL-8 as compared with the control serum in A549 cells, and the induced cytokine production was significantly suppressed by 15% serum containing Feiyanning Decoction （P〈0.01）. In addition, serum containing Feiyanning Decoction could inhibit the mRNA expressions of IL-6 and IL-8 （P〈0.01）. Conclusion： Feiyanning Decoction can inhibit IL-6 and IL-8 production induced by TNF-α. It is therefore expected to be a new strategy for treating lung cancer.