雷公藤治疗子痫前期孕鼠蛋白尿实验研究

Experimental Study on Polyglycosides of Tripterygium wilfordii on Proteinuria in A Rat Model of Preeclampsia

目的研究雷公藤多甙对于子痫前期孕鼠蛋白尿的治疗作用。方法采用皮下注射亚硝酸左旋精氨酸甲酯(LNAME)125mg·(kg·d)-1建立大鼠子痫前期模型。将造模后的大鼠随机分为3组:即大、小剂量治疗组和对照组。2个治疗组分别给予6mg·(kg·d)-1或3mg·(kg·d)-1灌胃,对照组给予0.9%氯化钠注射液灌胃。另设一健康大鼠对照组即D组。测量孕鼠尾动脉收缩压、蛋白尿浓度、肾功能、胎盘质量、仔鼠体长、体质量及肝、脑质量。结果与对照组相比,雷公藤多甙治疗组的尿蛋白明显降低,胎盘质量、仔鼠体长及体质量增加,仔鼠死亡率明显降低。3组之间畸形率无显著性差异。结论雷公藤多甙可以显著降低子痫前期孕鼠的蛋白尿水平,增加胎盘质量、仔鼠体长及体质量,降低死胎率,而未增加仔鼠的畸形率。

Objective To investigate the protective effects of polyglycosides from Tripterygium wilfordii（GTW） on proteinuria in a rat model of preeclampsia. Methods Preeelampsia was induced by subcutaneous injection of L-NAME, an inhibitor of nitric oxide synthase. Controls received injections of saline. GTW was administered through intragastric injection. Rats were randomly assigned to one of four treatment groups： group A received L-NAME and GTW[ 6 mg·（kg·d）^-1]; group B received L-NAME and GTW[ 3 mg· （kg·d） ^-1] ; group C received L-NAME and vehicle; group D received saline and vehicle. Systolic pressure（ SBP）, 24-hour urine protein （UP） were measured respectively. Blood samples were obtained for measuring creatinine and blood urea nitrogen. Newborn pups and placenta were weighed separately. Results Compared with group C, GTW significantly decreased the urine protein in pregnant rats. The ereatinine and blood urea nitrogen in the L-NAME-treated groups were significantly increased. But there was no significant difference among the three groups. Compared with group C, body length and weight were significantly increased in group A and B, whereas there was no significant difference between the two groups. The mortality and incidence of malformation was significantly increased in L-NAME- treated groups, while there was no significant difference among the three groups. Conclusion GTW could reduce the amount of proteinuria in L-NAME induced preeclampsia rats. No adverse effect of GTW was observed on newborn pups.