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寒冷应激对大鼠肾上腺髓质亨廷顿蛋白相关蛋白1表达的影响 被引量：1

Effect of cold stress on the expression of Huntingtin-associated protein 1 in the rat adrenal medulla
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摘要 目的探讨亨廷顿蛋白相关蛋白1（HAP1）在大鼠肾上腺髓质的超微结构定位,以及寒冷应激对大鼠肾上腺髓质HAP1表达的影响。方法成年雄性Wistar大鼠14只,2只用于免疫电镜研究,12只用于寒冷实验研究。寒冷实验中,将动物随机分为对照组和寒冷组,每组6只,寒冷组动物放置4℃环境下,12h后用免疫组织化学和Western blotting方法检测大鼠肾上腺髓质HAP1表达的变化。结果免疫电镜结果显示,HAP1免疫反应产物分布在肾上腺髓质细胞分泌颗粒外膜及分泌颗粒间的膜性细胞器上。寒冷组大鼠肾上腺髓质HAP1的表达明显减少,和对照组比较有显著性差异（P＆lt;0.01）。结论HAP1可能与肾上腺髓质细胞内分泌颗粒及位于分泌颗粒内的肾上腺素/去甲肾上腺素的运输和释放有关。 Objective To observe the ultrastructure location of Huntingtin-associated protein 1 （HAP1） in rat adrenal medulla and the effect of cold stress on the expression of HAP1 in rat adrenal medulla. Methods Fourteen healthy male Wistar rats were used in the present study and among them two rats were used for immune electron microscopy and twelve rats for cold stress experiment. In the cold stress experiment, animals were divided into control and cold groups randomly with six rats in each group. During the experiment, rats were housed in a room at the temperature of 4~C for 12 hours and then immunohistochemistry and Western blotting were used to measure the expression of HAP1 in adrenal medulla. Results By using the immune electron microscopy, the results showed HAP1 was located on the external membrane of secretory vesicles of adrenal medulla and their membranous organelles. The expression of HAP1 in adrenal medulla of cold group significantly decreased as compared with that of control group （ P 〈 0.01 ）. Conclusion HAP1 might be related with the adrenal medulla cells endocrine granula and involved in the transmission and release of adrenaline or noradrenaline in secretory granule.
作者 廖敏 张亦农 李和
机构地区 温州医学院组织学与胚胎学教研室 华中科技大学同济医学院组织学与胚胎学教研室
出处 《解剖学报》 CAS CSCD 北大核心 2009年第4期621-624,共4页 Acta Anatomica Sinica
基金 国家杰出青年科学基金资助项目(30225024) 国家自然科学基金资助项目(30671106)
关键词 亨廷顿蛋白相关蛋白1 寒冷 肾上腺髓质 免疫电镜 免疫印迹法 大鼠 Huntingtin-associated protein 1 Cold Adrenal medulla Immune electron microscopy Western blotting Rat
分类号 R322.56 [医药卫生—人体解剖和组织胚胎学]
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参考文献15

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  • 2Gutekunst CA, Li SH, Yi H, et al. The cellular and subcellular localization of huntingtin-associated protein 1 ( HAP1 ) : comparison with huntingtin in rat and human [J]. J Neurosci, 1998, 18(19): 7674-7686.
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同被引文献13

  • 1Li X J,Li S H, Sharp A H,et al. A huntingtin-associated protein enriched in brain with implications for pathology [ J ]. Nature, 1995,378 (6555) :398-402.
  • 2Gutekunst C A, Li S H, Yi H,et al. The cellular and subcellular lo- calization of huntingtin-associated protein 1 ( HAP1 ) : comparison with huntingtin in rat and human[ J]. Neuroscience, 1998,18 ( 19 ) : 7674-7686.
  • 3Martin E J, Kim M, Velier J,et al. Analysis of huntingtin-associated protein 1 in mouse brain and immortalized striatal neurons [ J ]. J Comp Neurol ,1999 , 403 ( 4 ) :421430.
  • 4Liao M, Shen X X, Han J H, et al. Selective expression of hunting- tin-associated protein 1 in B-cells of the rat pancreatic islets [ J ]. J Histochem Cytochem ,2010,58 ( 3 ) :255-263.
  • 5Li S H, Gutekunst C A, Hersch S M,et al. Interaction of hunting- tin-associated protein with dynactin P150Glued [ J ]. Neuroscience, 1998,18(4) :1261-1269.
  • 6Li S H, Li H,Tovre E R, et al. Expression of huntingtin-associated protein-1 in neuronal cells implicates a role in neuritic growth[J].Mol Cell Neurol, 2000,16 ( 2 ) : 168-183.
  • 7McGuire J R, Rong J, Li S H,et al. Interaction of huntingtin-associ- ated protein 1 with kinesin light chain:implications in intracellular trafficking in neurons [ J ]. J Biol Chem, 2006,281 ( 6 ) : 3552- 3559.
  • 8Block-Galarza J, Chase K O, Sapp E,et al. Fast transport and retro- grade movement of huntingtin and HAPI in axons [ J ]. Neuroreport, 1997,8(9/10) :2247-2251.
  • 9Rong J,Li S H, Li X J. Regulation of intracellular HAPI traffic- king[J].J Neurosci Res ,2007,85 ( 14 ) :3025-3029.
  • 10Sheng G Q,Xu X S,Lin Y F,et al. Huntingtin-associated protein 1 interacts with Ahil to regulate cerebellar and brainstem devel- opment in mice [ J ]. J Clin Invest,2008,118 ( 8 ) :2785-2795.

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解剖学报
2009年 第4期

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