生物样品中铜的石墨炉原子吸收检测及其在肝豆状核变性诊断中的应用

Determination of copper in biological samples by graphite furnace atomic absorption spectrometry and application in hepatolenticular degeneration diagnosing

目的建立诊断肝豆状核变性患者的生物样品中微量铜的石墨炉原子吸收检测方法。方法尿液和血清样品经1%HNO3稀释后,采用石墨炉原子吸收光谱标准曲线法直接测定。结果线性范围0～200μg/L,相关系数0.9997,方法检出限为1.4μg/L,回收率96.7%～99.2%,相对标准偏差1.0%～1.7%,经冻干人尿标样(GBW 09102)对照测定,其结果在允许范围内,相对误差为-1.2%。结论采用GFAAS法测定生物样品中铜,方法可靠、准确、灵敏,能适用于肝豆状核变性诊断中生物样品微量铜检测的要求。

Objective To establish a new method for determination of trace copper in biological samples of hepatolenticular degeneration patients by graphite furnace atomic absorption spectrometry （GFAAS）. Methods Urine and serum samples were diluted by 1% HNO3 , then the determination was directly performed with the standard curve method by GFAAS. Results The linear range was 0-200 μg/L, the correlation coefficient was 0. 9997, and the determination limit was 1.4 μg/L, the recovery rate was 96.7% - 99.2%, the relative standard deviation was 1.0% - 1.7%. In comparison with the determination in freeze dried urine reference material （GBW 09102）, the result by this method was in permit range, the relative error （n = 6） was - 1.2%. Conclusion GFAAS is quick, reliable, accurate and sensitive, which is suitable for the determination of trace copper in biological samples for hepatolenticular degeneration diagnosing.