小剂量纳洛酮对氯胺酮抗利多卡因惊厥作用的影响

Influence of low-dose Naloxone on anti-convulsion of Ketamine induced by lidocaine

目的:观察氯胺酮(KT)抗利多卡因(L)致惊厥作用的效果以及小剂量纳洛酮(NL)对此效应的影响。方法:昆明种小鼠按随机分层区组设计分组。(1)用序贯法测定预先皮下注射生理盐水(NS)或KT后L致惊厥ED50;(2)观察KT对L致惊小鼠惊厥持续期、惊厥次数、惊厥总时间和惊厥数的影响;(3)观察小剂量NL对L致惊厥作用的影响;(4)观察小剂量NL对KT抗L致惊厥作用的影响。结果:(1)NS组和KT组L致小鼠惊厥的ED50分别为62.95mg.kg-1和75.68mg.kg-1,差异无显著性(P>0.05);(2)与NS组比较,KT2组的惊厥持续期缩短(P<0.05),与KT1组比较,KT2组的惊厥次数减少(P<0.05),发作总时间有降低趋势,但差异无显著性(P>0.05);(3)与NS组比较,NL1000组的惊厥次数减少(P<0.05);(4)与NS组和NL组比较,NL+KT组惊厥持续期缩短(P<0.05),惊厥次数减少(P<0.01)。结论:在本实验条件下,较高剂量氯胺酮有较明显抗利多卡因惊厥作用,小剂量纳洛酮能增强此效应。

OBJECTIVE To observe the effect of low-dose Naloxone（NL）on anti-convulsion of ketamine（KT） induced by lidocaine （L）. METHODS Mice were randomly divided into groups. （1）To determine,with Sequence method, ED50, of L which can cause convulsion after hypodermic injection of NS or KT beforehand. （2）Observe the effect of KT on convulsion induced by L, the duration of tonic state, frequency, total time, incidence of convulsion were noted. （3）Observe the effect of NL on convulsion induced by L; （4）Observe the effect of low-dose NL on anti convulsion of KT induced by L. RESULTS （1） In NS group and KT group, the ED50 of L induced convulsions in mice were 62.95 mg·kg ^-1 and 75.68 mg·kg ^-1 , no statistically significant difference （P〉0. 05） ; （2）Compared with the NS group, The number of seizures, convulsions and duration of the seizures in KT2 group were a number of lower （P〈0. 05）and total time attacks had decreased, but the difference was not significant （P〉 0. 05） ; （3） Compared with the NS group, NL1000 group reduced the number of seizures （P〈0. 05） ; （4）Compared with the NS group and the NL group; NL ＋ KT group reduced the duration （P 〈0. 05） and the number of seizures （P 〈 0.01 ）. CONCLUSION In the experimental condition,a higher dose of ketamine has the effect of anti-lidocaine induced convulsion,and a small dose of naloxone enhanced this effect.