滋阴益气熄风活血通络法对糖尿病大鼠糖代谢及症状的实验研究

Experiment Study of Nourishing Yin,Invigorating Qi,Extinguishing Wind,Promoting Blood Circulation and Draining Collateral Method to Glucose Metabolism and Symptoms to DM Rats

目的:通过滋阴益气熄风活血通络法观察对糖尿病大鼠糖代谢及症状的影响。方法:将60只SD雄性大鼠随机分为正常组、模型组、西药组、降糖舒络方高剂量组、降糖舒络方中剂量组、降糖舒络方低剂量组6组,采用STZ腹腔注射,建立SD大鼠糖尿病模型。以实验大鼠的一般情况、血糖、体重、尿量、食量、饮水量为观察指标,以达美康为对照进行观察。结果:各组大鼠造模前饮水量、体重、食量、尿量、血糖均无明显改变(P>0.05);造模后各组大鼠的饮水量、体重、食量、尿量、血糖跟模型组相比均有显著差异(P<0.01);实验结束后西药组和中药组血糖较模型组均有明显改善,而对于饮水量、食量、尿量的改善中药组优于西药组,而且呈剂量依赖性。西药组对DM大鼠的体重及血糖影响优于中药组(P<0.01),中药各组对大鼠体重影响以中药大剂量组疗效占优(P<0.05),但是对血糖影响无组间差异(P>0.05)。结论:滋阴益气熄风活血通络法不是通过降低血糖改善糖尿病大鼠症状,其具体作用机制尚需进一步研究。

Objective： To observe the effect of nourishing Yin, invigorating Qi, promoting blood circulation, extinguishing wind and draining collateral method to the glucose metabolism and symptoms of DM rats. Methods：60 SD male rats were randomly divided normal group, model group, western medicine group, jiangtangshuluo fomula high dosage group, jiangtangshuluo formula middle dosage group, jiangtangshuluo formula low dosage group into 6 groups, had abdominal cavity injection by STZ to make SD rats DM model. Compared with gliclazide, took ordinary circumstances, BG, body weight, urine volume, appetite, drinking amount as observational targets. Results：There were no obviously changement of drinking amount, body weight, appetite, urine volume, BG of each group DM rats before making models （ P 〉 0.05 ）. There were obviously significance of drinking amount, body weight, appetite, urine volume, BG of each group DM rats after making models（ P 〈 0.01 ）, After the experiment ended, the ordinary circumstances and BG of the western medicine group and the TCM group had obviously changement compared with the model group, the effect of TCM group to drinking amount, appetite, urine volume was better than the western medicine group, and assumed the dosage dependence. The effect of body weight and BG of western medicine group DM rats restored better than the TCM group（ P 〈 0.01 ）, the curative effect of each TCM group compared, the high dosage group was better than the other two groups（ P 〈 0.05）, but there was no statistical significance among the TCM groups（ P 〉 0.05）. Conclusions：Nourishing Yin, invigorating Qi, extinguishing wind, promoting blood circulation and draining collateral method improve the symptoms was not by reducing BG, the concrete mechanism need further research. Key words： Nourishing Yin, invigorating Qi, promoting blood circulation, extinguishing wind and draining collateral method, DM rats, glucose metabolism and symptoms, Experiment study.